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Invitrogen
NEFL Monoclonal Antibody (1H3)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-17135
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1H3
Immunogen
Purified recombinant fragment of human NEFL expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Amount
100 µg
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17135 targets NEFL in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.
The MA5-17135 immunogen is purified recombinant fragment of human NEFL expressed in E. Coli.
MA5-17135 detects NEFL which has a predicted molecular weight of approximately 62kDa.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ53642; intermediate filament; light molecular weight neurofilament protein; N-FL; NEF3; NEFL; neurofilament; neurofilament 3; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110; unnamed protein product
Gene Aliases: CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Human) P07196
Entrez Gene ID: (Human) 4747
anterograde axonal transport
retrograde axonal transport
response to toxic substance
axonal transport of mitochondrion
spinal cord development
hippocampus development
cerebral cortex development
neurofilament bundle assembly
response to peptide hormone
intermediate filament polymerization or depolymerization
intermediate filament organization
protein polymerization
response to corticosterone
regulation of synapse maturation
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
response to sodium arsenite
response to acrylamide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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