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Invitrogen
SIAH2 Monoclonal Antibody (24E6H3)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-24780
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
24E6H3
Immunogen
Synthetic peptide made to an N-terminal portion of the drosophila SINA protein (within residues 1-100).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
Target Information
SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anon-fast-evolving-2H7; CG9949-PA; CG9949-PB; CG9949-PC; EC 6.3.2; HL08111p; mSiah2; RING-type E3 ubiquitin transferase SIAH2; seven in absenti; seven in absentia 2; seven in absentia homolog 2; seven-in-absentia; siah-2; sina-PA; sina-PB; sina-PC; unnamed protein product
Gene Aliases: anon-EST:fe2H7; CG9949; Dmel\CG9949; Dmel_CG9949; hSiah2; sia; SINA; Sinh2
UniProt ID: (Human) O43255, (Mouse) Q06986, (Fruit fly) P21461
Entrez Gene ID: (Human) 6478, (Pig) 100622531, (Mouse) 20439, (Fruit fly) 39884
ubiquitin-dependent protein catabolic process
small GTPase mediated signal transduction
protein ubiquitination
regulation of protein ubiquitination
regulation of circadian rhythm
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
amyloid fibril formation
regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway
protein catabolic process
sensory organ development
R7 cell fate commitment
proteasomal protein catabolic process
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
enteroendocrine cell differentiation
sevenless signaling pathway
regulation of R7 cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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