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Invitrogen
METTL3 Monoclonal Antibody (OTI1B7)
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FIGURE: 1 / 8
METTL3 Antibody (MA5-27527) in WB
Cell death discovery 2024 -
Fig. 5 METTL3 regulated the expression of SH3BGR in AC16 cells. A Depletion of METTL3 decreased abundance of m6A-modified SH3BGR mRNA in AC16 cells. B Overexpression of METTL3 induced SH3BGR mRNA m6A modification in AC16 cells. C RT-qPCR analysis of SH3BGR in control and METTL3 knockdown AC16 cells. D Western blotting analysis of METTL3 and SH3BGR with or without METTL3 knockdown in AC16 cells. E , F Statis... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27527
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1B7
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-284 of METTL3 produced in E.coli
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; m6a methyltransferase; methyltransferase like 3; methyltransferase-like 3; methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; N(6)-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit; N6-adenosine-methyltransferase subunit METTL3; unnamed protein product
Gene Aliases: 2310024F18Rik; hMETTL3; IME4; M6A; MT-A70; Spo8
UniProt ID: (Human) Q86U44, (Mouse) Q8C3P7
Entrez Gene ID: (Human) 56339, (Mouse) 56335, (Rat) 361035
mRNA splicing, via spliceosome
RNA methylation
mRNA processing
mRNA catabolic process
mRNA export from nucleus
cellular response to DNA damage stimulus
spermatogenesis
circadian rhythm
dosage compensation by inactivation of X chromosome
mRNA modification
stem cell population maintenance
forebrain radial glial cell differentiation
primary miRNA processing
cellular response to UV
gliogenesis
RNA stabilization
regulation of T cell differentiation
positive regulation of translation
negative regulation of Notch signaling pathway
oogenesis
regulation of meiotic cell cycle
negative regulation of type I interferon-mediated signaling pathway
positive regulation of mRNA catabolic process
mRNA destabilization
endothelial to hematopoietic transition
chromosomal DNA methylation maintenance following DNA replication
regulation of hematopoietic stem cell differentiation
positive regulation of cap-independent translational initiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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