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Invitrogen
NLRC4 Monoclonal Antibody (GT1387)
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FIGURE: 1 / 3
NLRC4 Antibody (MA5-27846) in ICC/IF
Immunofluorescence analysis of NLRC4 (NLR family CARD domain-containing protein 4) was performed using log phase THP-1 and Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NLRC4 Monoclonal Antibody (GT1387) (Product # MA5-27846) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Ant... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27846
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT1387
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human CARD12. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: human NLRC4-transfected 293T
Predicted Reactivity: Bovine (82%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NLRC4 encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARD protein; CARD, LRR, and NACHT-containing protein; CARD12; CARD12; CED4/Apaf-1 family member; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CED-4-like protein Clan; ICE-protease activating factor; IPAF; CED4/Apaf-1 family member; NOD-like receptor C4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4; unnamed protein product
Gene Aliases: AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
UniProt ID: (Human) Q9NPP4
Entrez Gene ID: (Human) 58484
activation of innate immune response
pattern recognition receptor signaling pathway
inflammatory response
positive regulation of protein processing
detection of bacterium
positive regulation of interleukin-1 beta production
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
icosanoid biosynthetic process
positive regulation of inflammatory response
protein homooligomerization
pyroptosis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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