Search
Invitrogen
USP7 Monoclonal Antibody (GT481)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 10
USP7 Antibody (MA5-31515) in ICC/IF
Immunofluorescence analysis of USP7 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with USP7 Monoclonal Antibody (GT481) (Product # MA5-31515, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:2,0... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-31515
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
GT481
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (93%), Pig (97%), Chicken (93%).
Positive Control: 293T, A431, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Chromosome 16p13.2 deletion syndrome; deubiquitinating enzyme 7; Herpes virus-associated ubiquitin-specific protease; herpes-virus-associated ubiquitin-specific protease; herpesvirus-associated ubiquitin-specific protease; mHAUSP; rHAUSP; ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7; ubiquitin specific protease 7 (herpes virus-associated); ubiquitin thioesterase 7; ubiquitin thiolesterase 7; ubiquitin-specific-processing protease 7; ubiquitinating enzyme; mHAUSP; unnamed protein product; USP7; herpes virus-associated protease
Gene Aliases: 2210010O09Rik; C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP; TEF1
UniProt ID: (Human) Q93009, (Rat) Q4VSI4, (Mouse) Q6A4J8
Entrez Gene ID: (Human) 7874, (Rat) 360471, (Mouse) 252870
transcription-coupled nucleotide-excision repair
DNA dealkylation involved in DNA repair
regulation of tumor necrosis factor-mediated signaling pathway
protein deubiquitination
regulation of protein stability
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
monoubiquitinated protein deubiquitination
regulation of circadian rhythm
positive regulation of apoptotic process
hypermethylation of CpG island
negative regulation of gluconeogenesis
protein stabilization
regulation of establishment of protein localization to telomere
protein K63-linked deubiquitination
protein K48-linked deubiquitination
symbiont-mediated disruption of host cell PML body
antiviral innate immune response
transcription elongation-coupled chromatin remodeling
negative regulation of TORC1 signaling
regulation of retrograde transport, endosome to Golgi
protein ubiquitination
regulation of signal transduction by p53 class mediator
regulation of telomere capping
autophagosome assembly
negative regulation of autophagy
positive regulation of autophagy
cellular response to nutrient levels
TORC1 signaling
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support