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Invitrogen
FANCD2 Recombinant Rabbit Monoclonal Antibody (SN73-08)
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FIGURE: 1 / 9
FANCD2 Antibody (MA5-32316) in ICC/IF
Knockout of FANCD2 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR718017_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on wild type HeLa cells (panel a,d), HeLa Cas9 cells (panel b, e) and HeLa FANCD2 KO cells (panel c,f). Cells were fixed, permeabilized, and labelled with FANCD2 Recombinant Rabbit Monoclonal Antibody (SN73-08) (Product # MA5-32316, 1:100 dilution), followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbe... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-32316
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SN73-08
Immunogen
Synthetic peptide within Human FANCD2 aa 191-240.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
FANCD2 (Fanconi anemia group D2 protein) is required for maintenance of chromosomal stability. It promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-stranded breaks, both by homologous recombination and single-stranded annealing. It is required for the targeting/stabilization of BLM to non-centromeric abnormal structures induced by replicative stress. FANCD2 promotes BRCA2/FANCD1 loading onto damaged chromatin. Defects in the FANCD2 gene result in Fanconi anemia complementation group D2. This disorder affects all bone marrow elements resulting in anemia, leukopenia, and thrombopenia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp762A223; FACD2; Fanconi anemia complementation group D2; Fanconi anemia D2 protein; Fanconi anemia, complementation group D2; FLJ23826; unnamed protein product
Gene Aliases: 2410150O07Rik; FA-D2; FA4; FACD; FAD; FAD2; FANCD
UniProt ID: (Human) Q9BXW9, (Rat) Q6IV68, (Mouse) Q80V62
Entrez Gene ID: (Human) 2177, (Rat) 312641, (Mouse) 211651
DNA repair
synapsis
response to gamma radiation
intra-S DNA damage checkpoint
interstrand cross-link repair
double-strand break repair involved in meiotic recombination
cellular response to DNA damage stimulus
gamete generation
biological_process
cellular response to oxidative stress
regulation of regulatory T cell differentiation
brain morphogenesis
regulation of inflammatory response
neuronal stem cell population maintenance
regulation of CD40 signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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