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Glucocorticoid Receptor Recombinant Rabbit Monoclonal Antibody (JF0952)

View all (70) Glucocorticoid Receptor (Nr3c1) antibodies

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Glucocorticoid Receptor Recombinant Rabbit Monoclonal Antibody (JF0952)

Product Details

MA5-32503

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JF0952

Immunogen

Recombinant protein within Human Glucocorticoid Receptor aa 2-220.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809780

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor subtype P; GR; gr alpha; GR Kit; hGR DL-1; hGR NS-1; nuclear receptor subfamily 3 group C member 1 variant hGR-B(54); nuclear receptor subfamily 3 group C member 1 variant hGR-B(77); nuclear receptor subfamily 3 group C member 1 variant hGR-B(93); nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); nuclear receptor; polymorphic form; OTTHUMP00000222854; OTTHUMP00000222858; putative upstream ORF.; Unknown; unnamed protein product

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Gene Aliases: fb13f09; GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; utouto; utut; wu:fb13f09; zgc:113038

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UniProt ID: (Human) P04150, (Rat) P06536, (Mouse) P06537

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Entrez Gene ID: (Human) 2908, (Rat) 24413, (Mouse) 14815, (Zebrafish) 553740

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding glucocorticoid receptor activity steroid binding protein binding zinc ion binding TBP-class protein binding protein kinase binding estrogen response element binding identical protein binding sequence-specific DNA binding Hsp90 protein binding steroid hormone binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding chromatin binding double-stranded DNA binding transcription coactivator activity Hsp70 protein binding receptor tyrosine kinase binding heat shock protein binding hormone binding macromolecular complex binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter positive regulation of cytokine production regulation of gluconeogenesis chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter female pregnancy circadian rhythm glucocorticoid metabolic process associative learning response to wounding gene expression regulation of glucose metabolic process microglia differentiation positive regulation of glutamate secretion response to activity response to inactivity muscle atrophy lung development adrenal gland development intracellular steroid hormone receptor signaling pathway negative regulation of synaptic plasticity regulation of glucocorticoid biosynthetic process nuclear receptor-mediated corticosteroid signaling pathway response to insulin Leydig cell differentiation synaptic transmission, glutamatergic regulation of cell proliferation maternal behavior glucocorticoid receptor signaling pathway negative regulation of apoptotic process negative regulation of vascular permeability positive regulation of neuron apoptotic process positive regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to arsenic-containing substance response to mercury ion astrocyte differentiation response to glucocorticoid response to corticosterone response to cortisol response to calcium ion response to electrical stimulus mammary gland duct morphogenesis positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development motor behavior protein-containing complex assembly cellular response to magnesium ion cellular response to steroid hormone stimulus cellular response to glucocorticoid stimulus response to dexamethasone cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus response to alcohol neuroinflammatory response negative regulation of long term synaptic depression response to ketone positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of nuclear receptor-mediated glucocorticoid signaling pathway negative regulation of glucocorticoid receptor signaling pathway positive regulation of glucocorticoid receptor signaling pathway negative regulation of behavioral fear response signal transduction motor neuron apoptotic process regulation of metabolic process multicellular organismal response to stress light adaption chordate embryonic development glucocorticoid mediated signaling pathway circadian behavior embryonic viscerocranium morphogenesis ion homeostasis calcium ion homeostasis sodium ion homeostasis calcium ion import proton export across plasma membrane regulation of hematopoietic stem cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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