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Invitrogen
SF3B1 Recombinant Rabbit Monoclonal Antibody (JB40-32)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-34649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JB40-32
Immunogen
Recombinant protein within Human SF3B1 aa 1-105.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Mouse thymus tissue lysate, Hela, human colon cancer tissue, human skin tissue, human kidney tissue, mouse small intestine tissue.
Target Information
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex. The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: pre-mRNA processing 10; pre-mRNA splicing factor SF3b, 155 kDa subunit; similar to Xenopus laevis 146 kDa nuclear protein with GenBank Accession Number Y08997; spliceosome-associated protein 155; splicing factor 3b, subunit 1, 155kDa; unnamed protein product
Gene Aliases: Hsh155; MDS; PRP10; PRPF10; SAP155; SF3b155
UniProt ID: (Human) O75533
Entrez Gene ID: (Human) 23451
spliceosomal complex assembly
RNA splicing, via transesterification reactions
spliceosomal snRNP assembly
spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
mRNA splicing, via spliceosome
chromatin remodeling
RNA splicing
mRNA cis splicing, via spliceosome
positive regulation of transcription from RNA polymerase I promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
U2-type prespliceosome assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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