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Invitrogen
UBC9 Recombinant Rabbit Monoclonal Antibody (6Z9V6)
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FIGURE: 1 / 5
UBC9 Antibody (MA5-35505) in IHC (P)
Immunohistochemistry analysis of UBC9 in paraffin-embedded human colon carcinoma. Samples were incubated with UBC9 Monoclonal antibody (Product # MA5-35505) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35505
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6Z9V6
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2I (P63279)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.55 mg/mL
Amount
55 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mUBC9; OTTHUMP00000198131; RAD6 homolog; May be involved in ubiquitin conjugation; Interacts with RAD52 and RAD51 proteins; Method: conceptual translation supplied by author.; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-protein ligase; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; ubiquitin-protein ligase I; unnamed protein product
Gene Aliases: 5830467E05Rik; C358B7.1; P18; UBC9; UbcE2A; Ubce2i; Ubce9
UniProt ID: (Human) P63279, (Rat) P63281, (Mouse) P63280
Entrez Gene ID: (Human) 7329, (Rat) 25573, (Mouse) 22196
transcription cofactor binding
RNA binding
protein binding
transcription factor binding
SUMO transferase activity
enzyme binding
HLH domain binding
small protein activating enzyme binding
SUMO conjugating enzyme activity
RING-like zinc finger domain binding
ubiquitin-protein transferase activity
ionotropic glutamate receptor binding
bHLH transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
ubiquitin-dependent protein catabolic process
regulation of receptor activity
protein sumoylation
positive regulation of cell migration
positive regulation of intracellular steroid hormone receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
modulation of synaptic transmission
nuclear export
nuclear import
protein modification process
mitotic nuclear envelope reassembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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