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Invitrogen
BAG3 Recombinant Rabbit Monoclonal Antibody (8P3W7)
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FIGURE: 1 / 3
BAG3 Antibody (MA5-35563) in IHC (P)
Immunohistochemistry analysis of BAG3 in paraffin-embedded mouse kidney. Samples were incubated with BAG3 Monoclonal antibody (Product # MA5-35563) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35563
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8P3W7
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BAG3 (O95817)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: NDPRVPSEGP KETPSSANGP SREGSRLPPA REGHPVYPQL RPGYIPIPVL HEGAENRQVH PFHVYPQPGM QRFRTEAAAA APQRSQSPLR GMPETTQPDK Q
Target Information
BAG3 are among BAG proteins that compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BAG-3; Bcl-2 interacting cell death suppressor; bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Bcl-2-binding protein BIS transcript 2; Bcl-2-interacting death suppressor; BCL2 associated athanogene 3; BCL2-binding athanogene 3; DKFZp434E0610; docking protein CAIR-1; MGC104307; unnamed protein product
Gene Aliases: BAG-3; BIS; CAIR-1; CMD1HH; CMT2JJ; HMND15; MFM6; mg638
UniProt ID: (Human) O95817, (Mouse) Q9JLV1
Entrez Gene ID: (Human) 9531, (Mouse) 29810
autophagosome assembly
protein folding
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of striated muscle cell apoptotic process
spinal cord development
cellular response to heat
cellular response to unfolded protein
positive regulation of protein import into nucleus
negative regulation of apoptotic process
muscle cell cellular homeostasis
positive regulation of protein export from nucleus
protein stabilization
chaperone-mediated autophagy
aggresome assembly
extrinsic apoptotic signaling pathway
protein transport along microtubule
negative regulation of establishment of protein localization to mitochondrion
positive regulation of aggrephagy
cellular response to mechanical stimulus
extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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