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Invitrogen
Annexin V Recombinant Rabbit Monoclonal Antibody (9A5M8)
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FIGURE: 1 / 3
Annexin V Antibody (MA5-35789) in WB
Western blot analysis of Annexin V in extracts of various cell lines. Samples were incubated with Annexin V Monoclonal antibody (Product # MA5-35789) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35789
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9A5M8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 221-320 of human Annexin A5/Annexin A5/Annexin V (NP_0011451)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: IEETIDRETS GNLEQLLLAV VKSIRSIPAY LAETLYYAMK GAGTDDHTLI RVMVSRSEID LFNIRKEFRK NFATSLYSMI KGDTSGDYKK ALLLLCGEDD
Target Information
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: anchorin CII; Annexin V; annexin-5; anticoagulant precursor (5' end put.); putative; ANXA5; calphobindin I; CBP-I; endonexin II; epididymis secretory protein Li 7; lipocortin V; lipocortin-V; PAP-I; placental anticoagulant protein 4; placental anticoagulant protein I; PP4; thromboplastin inhibitor; unnamed protein product; VAC-alpha; vascular anticoagulant-alpha
Gene Aliases: ANX5; CPB-I; ENX2; HEL-S-7; LC5; PP4; RPRGL3; VAC-alph
UniProt ID: (Human) P08758, (Rat) P14668, (Mouse) P48036
Entrez Gene ID: (Human) 308, (Rat) 25673, (Mouse) 11747
signal transduction
negative regulation of apoptotic process
negative regulation of coagulation
negative regulation of blood coagulation
positive regulation of apoptotic process
response to calcium ion
calcium ion transmembrane transport
cellular response to lead ion
response to thyroid hormone
cellular response to gonadotropin-releasing hormone
regulation of sperm motility
negative regulation of prolactin secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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