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          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Invitrogen

          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (HL173)

          1 Reference
          View all (107) ERK1/2 antibodies

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          Cite Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (HL173)

          Additional Information:
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          • Antibody Testing Data (4)
          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Antibody (MA5-36265) in WB

          Western Blot using Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Monoclonal Antibody (HL173) (Product # MA5-36265). Untreated (–) and treated (+) Rat2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Monoclonal Antibody (HL173) (Product # MA5-36265) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG anti... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (HL173)

          Product Details

          MA5-36265

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Fruit fly, Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          HL173

          Immunogen

          Carrier-protein conjugated synthetic peptide surrounding phospho Thr202/Tyr204 of human ERK1. The exact sequence is proprietary.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2890401

          Product Specific Information

          Positive control: Neuro2A (50 µM Ethacrynic acid treatment for 2 hr) , A431 (starvation for 16 hr) , A431 (starvation for 16 hr and 200 ng/mL EGF treatment for 0.5 hr).

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; dp-ERK; dpERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-2; ERK/rolled; erk1 erk2; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; extracellular signal-regulated kinase; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; insulin-stimulated MAP2 kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP-kinase; MAPK; MAPK 1; MAPK 2; MAPK 3; microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen-activated 3; mitogen-activated protein kinase; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; phospho-ERK; pp42/MAP kinase; protein serine/threonine kinase ERK1b; protein tyrosine kinase ERK2; RE08694p; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; sevenmaker; unnamed protein product

          View more View less

          Gene Aliases: 12559; 9030612K14Rik; BcDNA:RE08694; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmERK; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK1/2; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; GroupII; HS44KDAP; HUMKER1A; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; MAPK2; MNK1; Mtap2k; NS13; p-ERK; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B

          View more View less

          UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708, (Fruit fly) P40417

          View more View less

          Entrez Gene ID: (Human) 5594, (Human) 5595, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Fruit fly) 3354888

          View more View less

          Function(s)
          phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding double-stranded DNA binding protein kinase binding mitogen-activated protein kinase kinase kinase binding scaffold protein binding transcription factor binding non-receptor serine/threonine protein kinase
          Process(es)
          MAPK cascade regulation of transcription from RNA polymerase II promoter protein phosphorylation apoptotic process chemotaxis signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway chemical synaptic transmission learning or memory insulin receptor signaling pathway positive regulation of macrophage chemotaxis positive regulation of peptidyl-threonine phosphorylation Schwann cell development positive regulation of telomere maintenance regulation of stress-activated MAPK cascade cellular response to amino acid starvation response to nicotine intracellular signal transduction ERBB signaling pathway ERBB2-ERBB3 signaling pathway myelination positive regulation of skeletal muscle tissue regeneration positive regulation of cholesterol biosynthetic process positive regulation of smoothened signaling pathway insulin-like growth factor receptor signaling pathway stress-activated MAPK cascade regulation of cytoskeleton organization interleukin-34-mediated signaling pathway chemokine-mediated signaling pathway ERK1 and ERK2 cascade response to epidermal growth factor caveolin-mediated endocytosis regulation of Golgi inheritance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response regulation of early endosome to late endosome transport negative regulation of T cell mediated immune response to tumor cell phosphorylation sensory perception of pain BMP signaling pathway positive regulation of cyclase activity peptidyl-tyrosine autophosphorylation signal transduction in response to DNA damage positive regulation of transcription from RNA polymerase II promoter modulation of synaptic transmission positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus negative regulation of cholesterol efflux negative regulation of TORC1 signaling cellular response to DNA damage stimulus heart development positive regulation of cell proliferation response to toxic substance organ morphogenesis neural crest cell development diadenosine tetraphosphate biosynthetic process peptidyl-serine phosphorylation cytosine metabolic process regulation of ossification positive regulation of cell migration thyroid gland development lipopolysaccharide-mediated signaling pathway response to estradiol response to lipopolysaccharide response to testosterone mammary gland epithelial cell proliferation cellular response to platelet-derived growth factor stimulus response to cocaine positive regulation of protein import into nucleus outer ear morphogenesis response to hydrogen peroxide response to exogenous dsRNA response to estrogen estrous cycle negative regulation of cell differentiation positive regulation of translation positive regulation of transcription, DNA-templated decidualization thymus development T cell receptor signaling pathway B cell receptor signaling pathway regulation of intracellular pH Bergmann glial cell differentiation positive regulation of cardiac muscle cell proliferation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development cellular response to methionine cellular response to cAMP cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus cellular response to peptide hormone stimulus cellular response to prostaglandin E stimulus cellular response to lipid cellular response to toxic substance response to alcohol cellular response to insulin-like growth factor stimulus placenta development autophagosome assembly negative regulation of autophagy positive regulation of autophagy cellular response to nutrient levels negative regulation of proteasomal ubiquitin-dependent protein catabolic process TORC1 signaling protein stabilization negative regulation of T cell activation cartilage development protein-containing complex assembly positive regulation of xenophagy arachidonic acid metabolic process mitotic cell cycle terminal region determination terminal branching, open tracheal system R7 cell fate commitment imaginal disc-derived wing vein specification imaginal disc-derived wing morphogenesis leg disc proximal/distal pattern formation metamorphosis torso signaling pathway determination of adult lifespan fibroblast growth factor receptor signaling pathway cellular response to starvation regulation of gene expression negative regulation of macroautophagy cellular response to reactive oxygen species lymph gland plasmatocyte differentiation lymph gland crystal cell differentiation tracheal pit formation in open tracheal system sevenless signaling pathway positive regulation of cell size positive regulation of photoreceptor cell differentiation dorsal appendage formation vascular endothelial growth factor receptor signaling pathway behavioral response to ethanol positive regulation of sequence-specific DNA binding transcription factor activity defense response to virus branching involved in open tracheal system development cellular response to arsenic-containing substance cellular response to cadmium ion positive regulation of wound healing regulation of heart morphogenesis regulation of response to drug
          It has to be done as per old AB suggested Products section.

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