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Invitrogen
Hex Recombinant Rabbit Monoclonal Antibody (9P4E4)
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FIGURE: 1 / 1
Hex Antibody (MA5-37951) in WB
Western blot analysis of Hex in extracts of HepG2 cells. Samples were incubated with Hex Monoclonal antibody (Product # MA5-37951) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-37951
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9P4E4
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hex (Q03014)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.46 mg/mL
Amount
46 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HepG2
Immunogen sequence: TLPSPNSSFT SLVSPYRTPV YEPTPIHPAF SHHSAAALAA AYGPGGFGGP LYPFPRTVND YTHALLRHDP LGKPLLWSPF LQRPLHKRKG GQVRFSNDQT I
Target Information
HHEX is a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation. Establishes anterior identity at two levels 1) acts early to enhance canonical WNT-signaling by repressing expression of TLE4 and 2) acts later to inhibit NODAL-signaling by directly targeting NODAL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homeobox; homeobox protein HEX; homeobox protein PRH; homeobox, hematopoietically expressed; huprobox; proline-rich homeodomain protein; proline-rich homeodomain-containing transcription factor; unnamed protein product
Gene Aliases: HEX; HMPH; HOX11L-PEN; PRH; PRHX
UniProt ID: (Human) Q03014
Entrez Gene ID: (Human) 3087
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
eukaryotic initiation factor 4E binding
DNA binding, bending
TBP-class protein binding
protein homodimerization activity
sequence-specific DNA binding
translation regulator activity
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
anterior/posterior pattern specification
regulation of mRNA export from nucleus
negative regulation of angiogenesis
cell differentiation
positive regulation of Wnt signaling pathway
B cell differentiation
negative regulation of vascular endothelial growth factor receptor signaling pathway
protein localization to nucleus
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of leukocyte proliferation
DNA conformation change
positive regulation of canonical Wnt signaling pathway
negative regulation of cytoplasmic translational initiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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