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          • Primary Antibodies ›
          • SEC61 alpha Antibodies

          Invitrogen

          SEC61alpha Recombinant Rabbit Monoclonal Antibody (9W7I3)

          View all (6) SEC61 alpha antibodies

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          Cite SEC61alpha Recombinant Rabbit Monoclonal Antibody (9W7I3)

          Additional Information:
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          • Antibody Testing Data (1)
          SEC61alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SEC61alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          SEC61alpha Antibody (MA5-38005) in WB

          Western blot analysis of SEC61alpha in extracts of various cell lines. Samples were incubated with SEC61alpha Monoclonal antibody (Product # MA5-38005) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SEC61alpha Antibody in Western Blot (WB)
          SEC61alpha Recombinant Rabbit Monoclonal Antibody (9W7I3)

          Product Details

          MA5-38005

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:6,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat, Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          9W7I3

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Sec61A (Q9H9S3)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.2 mg/mL

          Amount

          120 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 0.05% BSA, 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2897923

          Product Specific Information

          Positive Samples: 293T, SH-SY5Y, Mouse lung, Mouse testis, Mouse brain, Rat testis

          Immunogen sequence: SMGAIFEDPV HVVVYIIFML GSCAFFSKTW IEVSGSSAKD VAKQLKEQQM VMRGHRDTSM VHELNRYIPT AAAFGGLCIG ALSVLADFLG AIGSGTGILL A

          Target Information

          The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site. This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: protein transport protein Sec61 subunit alpha; Protein transport protein Sec61 subunit alpha isoform 2; Sec61 alpha; Sec61 alpha 2 subunit; Sec61 alpha form 2; Sec61 alpha-2; SEC61 translocon alpha 2 subunit; Sec61, alpha subunit 2; unnamed protein product

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          Gene Aliases: SEC61A2

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          UniProt ID: (Human) Q9H9S3, (Mouse) Q9JLR1

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          Entrez Gene ID: (Human) 55176, (Rat) 361273, (Mouse) 57743

          View more View less

          Function(s)
          signal sequence binding protein binding protein transmembrane transporter activity ribosome binding molecular_function transporter
          Process(es)
          SRP-dependent cotranslational protein targeting to membrane, translocation protein transport posttranslational protein targeting to membrane, translocation biological_process transport
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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