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Invitrogen
Phospho-Paxillin (Tyr118) Recombinant Rabbit Monoclonal Antibody (2E6P0)
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FIGURE: 1 / 1
Phospho-Paxillin (Tyr118) Antibody (MA5-38238) in WB
Western blot analysis of Phospho-Paxillin (Tyr118) in extracts of various cell lines. Samples were incubated with Phospho-Paxillin (Tyr118) Monoclonal antibody (Product # MA5-38238) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. A-431 cells were treated by pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight. NIH/3T... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-38238
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2E6P0
Immunogen
A synthetic phosphorylated peptide around Y118 of human Paxillin (P49023)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.93 mg/mL
Amount
93 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-431, NIH/3T3, C6
Immunogen sequence: HVYSF
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ16691; hCG_1778014; inverted formin-2-like; MIP7; myocardial ischemic preconditioning associated protein 7; PAX-1; PAXI; paxillin alpha; testicular tissue protein Li 134; unnamed protein product
Gene Aliases: Pax
UniProt ID: (Human) P49023, (Rat) Q66H76, (Mouse) Q8VI36
Entrez Gene ID: (Human) 5829, (Rat) 360820, (Mouse) 19303
MAPK cascade
cytoskeleton organization
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
regulation of cell shape
cell migration
peptidyl-tyrosine phosphorylation
lamellipodium assembly
lipopolysaccharide-mediated signaling pathway
substrate adhesion-dependent cell spreading
cellular response to reactive oxygen species
endothelial cell migration
positive regulation of angiogenesis
positive regulation of protein kinase activity
focal adhesion assembly
branching morphogenesis of an epithelial tube
positive regulation of stress fiber assembly
growth hormone receptor signaling pathway
positive regulation of substrate adhesion-dependent cell spreading
response to peptide
cell adhesion
signal transduction
signal complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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