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          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          SRC Recombinant Rabbit Monoclonal Antibody (JF0947)

          View all (81) SRC antibodies

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          Cite SRC Recombinant Rabbit Monoclonal Antibody (JF0947)

          Additional Information:
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          • Antibody Testing Data (11)
          SRC Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SRC Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          SRC Antibody (MA5-41202) in ICC/IF

          Immunocytochemistry/Immunofluorescence analysis of SRC in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with SRC Recombinant Monoclonal Antibody (Product # MA5-41202) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          SRC Antibody in Immunocytochemistry (ICC/IF)
          SRC Antibody in Immunocytochemistry (ICC/IF)
          SRC Antibody in Immunocytochemistry (ICC/IF)
          SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SRC Antibody in Western Blot (WB)
          SRC Antibody in Western Blot (WB)
          SRC Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          SRC Recombinant Rabbit Monoclonal Antibody (JF0947)

          Product Details

          MA5-41202

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:5,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -

          Flow Cytometry (Flow)

          1:50-1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          JF0947

          Immunogen

          Synthetic peptide within human Src aa 20-60.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 40% glycerol, 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2898956

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: c-Src; EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-src; pp60src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; Rous sarcoma oncogene; RP5-823N20.1; tyrosine kinase pp60c-src; tyrosine protein kinase c-src; tyrosine protein kinase pp60-c-src; tyrosine-protein kinase SRC-1; tyrosine-specific protein kinase; unnamed protein product; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

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          Gene Aliases: ASV; c-SRC; p60-Src; pp60c-src; SRC1; THC6

          View more View less

          UniProt ID: (Human) P12931, (Mouse) P05480, (Rat) Q9WUD9

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          Entrez Gene ID: (Human) 6714, (Mouse) 20779, (Rat) 83805

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          Function(s)
          protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding integrin binding protein binding ATP binding phospholipase activator activity kinase activity enzyme binding protein domain specific binding heme binding protein tyrosine kinase activator activity receptor activator activity ionotropic glutamate receptor binding SH2 domain binding phospholipase binding ion channel binding cadherin binding ephrin receptor binding ATPase binding phosphoprotein binding BMP receptor binding connexin binding scaffold protein binding protein kinase C binding insulin receptor binding protein kinase binding estrogen receptor binding macromolecular complex binding cell adhesion molecule binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter primary ovarian follicle growth positive regulation of cytokine production protein phosphorylation cell adhesion epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway gamete generation lactation positive regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of glucose metabolic process positive regulation of protein processing positive regulation of smooth muscle cell migration cell migration protein deubiquitination peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion cell differentiation positive regulation of cell migration forebrain development negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of telomere maintenance regulation of intracellular estrogen receptor signaling pathway toll-like receptor 3 signaling pathway regulation of toll-like receptor 3 signaling pathway substrate adhesion-dependent cell spreading cellular response to reactive oxygen species positive regulation of Rac protein signal transduction positive regulation of dephosphorylation negative regulation of hippo signaling intracellular signal transduction osteoclast development cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation angiotensin-activated signaling pathway TORC1 signaling vasodilation odontogenesis positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of MAP kinase activity transcytosis bone resorption positive regulation of Notch signaling pathway positive regulation of bone resorption positive regulation of cell adhesion positive regulation of cell size positive regulation of glycolytic process positive regulation of Ras protein signal transduction positive regulation of insulin receptor signaling pathway neurotrophin TRK receptor signaling pathway focal adhesion assembly oogenesis positive regulation of peptidyl-tyrosine phosphorylation progesterone receptor signaling pathway positive regulation of small GTPase mediated signal transduction positive regulation of protein transport negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly intestinal epithelial cell development interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly DNA biosynthetic process regulation of heart rate by cardiac conduction positive regulation of canonical Wnt signaling pathway positive regulation of wound healing positive regulation of protein localization to nucleus positive regulation of intracellular signal transduction negative regulation of neutrophil activation positive regulation of cellular protein localization negative regulation of TORC1 signaling positive regulation of TORC1 signaling cellular response to angiotensin positive regulation of vascular smooth muscle cell proliferation positive regulation of neural precursor cell proliferation positive regulation of male germ cell proliferation positive regulation of ovarian follicle development positive regulation of lamellipodium morphogenesis positive regulation of platelet-derived growth factor receptor-beta signaling pathway regulation of early endosome to late endosome transport negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway regulation of caveolin-mediated endocytosis spermatogenesis learning or memory response to xenobiotic stimulus response to mechanical stimulus response to acidic pH skeletal muscle cell proliferation response to nutrient levels cellular response to insulin stimulus response to ATP adherens junction organization response to hydrogen peroxide platelet-derived growth factor receptor signaling pathway behavioral response to cocaine response to mineralocorticoid myoblast proliferation response to electrical stimulus response to fatty acid cellular response to lipopolysaccharide cellular response to fatty acid cellular response to hypoxia cell-cell adhesion positive regulation of outer hair cell apoptotic process cellular response to prolactin stimulatory C-type lectin receptor signaling pathway negative regulation of inflammatory response to antigenic stimulus signal transduction signal complex assembly macroautophagy platelet activation osteoclast differentiation T cell costimulation positive regulation of integrin activation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway regulation of vascular permeability regulation of bone resorption viral entry into host cell vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway leukocyte migration
          It has to be done as per old AB suggested Products section.

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