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Invitrogen
OTX1 Recombinant Rabbit Monoclonal Antibody (6H7J9)
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FIGURE: 1 / 1
OTX1 Antibody (MA5-42732) in WB
Western blot analysis of OTX1 in extracts of various cell lines. Samples were incubated with OTX1 Monoclonal antibody (Product # MA5-42732) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42732
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H7J9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human OTX1 (NP_0553771)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Mouse brain, Rat brain.
Immunogen sequence: MMSYLKQPPY GMNGLGLAGP AMDLLHPSVG YPATPRKQRR ERTTFTRSQL DVLEALFAKT RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQSG
Target Information
Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homeobox protein OTX1-like; homeodomain 159..341; orthodenticle homolog 1; OTX; Otx1; unnamed protein product
Gene Aliases: A730044F23Rik; jv; OTX1X
UniProt ID: (Rat) Q63410, (Mouse) P80205
Entrez Gene ID: (Rat) 25646, (Mouse) 18423
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific double-stranded DNA binding
protein binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
anterior/posterior pattern specification
metencephalon development
forebrain development
midbrain development
inner ear morphogenesis
positive regulation of transcription from RNA polymerase II promoter
diencephalon morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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