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Invitrogen
MMP12 Recombinant Rabbit Monoclonal Antibody (1O3A4)
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FIGURE: 1 / 1
MMP12 Antibody (MA5-42836) in WB
Western blot analysis of MMP12 in extracts of various cell lines. Samples were incubated with MMP12 Monoclonal antibody (Product # MA5-42836) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42836
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1O3A4
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 371-470 of human MMP12 (P39900).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: A-549, BxPC-3, Mouse lung, Mouse brain, Mouse spleen, Rat lung. The target is usually found in the following locations: Secreted, extracellular matrix, extracellular space.
Immunogen sequence: FGFPNFVKKI DAAVFNPRFY RTYFFVDNQY WRYDERRQMM DPGYPKLITK NFQGIGPKID AVFYSKNKYY YFFQGSNQFE YDFLLQRITK TLKSNSWFGC
Target Information
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3.4.24.65; macrophage elastase; matrix metallo protease; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; MGC138506; MMP; MMP-12; Mmp12; MMPs; unnamed protein product
Gene Aliases: HME; ME; MME; Mmel; MMP-12
UniProt ID: (Human) P39900, (Rat) Q63341, (Mouse) P34960
Entrez Gene ID: (Human) 4321, (Rat) 117033, (Mouse) 17381
negative regulation of transcription from RNA polymerase II promoter
proteolysis
protein import into nucleus
positive regulation of gene expression
extracellular matrix disassembly
extracellular matrix organization
collagen catabolic process
positive regulation of interferon-alpha production
wound healing, spreading of epidermal cells
positive regulation of transcription from RNA polymerase II promoter
regulation of defense response to virus by host
positive regulation of epithelial cell proliferation involved in wound healing
elastin catabolic process
negative regulation of type I interferon-mediated signaling pathway
positive regulation of type I interferon-mediated signaling pathway
cellular response to virus
response to amyloid-beta
negative regulation of endothelial cell-matrix adhesion via fibronectin
response to hypoxia
response to xenobiotic stimulus
lung alveolus development
positive regulation of epithelial cell proliferation
regulation of trophoblast cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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