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          • Primary Antibodies ›
          • AKT Pan Antibodies

          Invitrogen

          Phospho-AKT Pan (Thr308, Thr305, Thr309) Recombinant Rabbit Monoclonal Antibody (5E9G5)

          1 Reference
          View all (62) AKT Pan antibodies

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          Cite Phospho-AKT Pan (Thr308, Thr305, Thr309) Recombinant Rabbit Monoclonal Antibody (5E9G5)

          Additional Information:
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          • Antibody Testing Data (1)
          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody (MA5-42960) in WB

          Western blot analysis of Phospho-AKT Pan (Thr308, Thr305, Thr309) in extracts of various cell lines. Samples were incubated with Phospho-AKT Pan (Thr308, Thr305, Thr309) Monoclonal antibody (Product # MA5-42960) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          MA5-42960

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 1 publication 1 publication

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          5E9G5

          Immunogen

          Synthetic peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.95 mg/mL

          Amount

          95 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2912101

          Product Specific Information

          Positive test controls include: NIH/3T3, C6.

          Target Information

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AKT1 kinase; Akt1m protein; Akt2; AKT2 kinase; AKT3 kinase; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; murine thymoma viral (v-akt) oncogene homolog 2; pan akt; PKB alpha; PKB beta; PKB gamma; PKBgamma/Akt3; protein kinase Akt-2; protein kinase Akt-3; protein kinase B alpha; protein kinase B beta; protein kinase B gamma; protein kinase B, beta; protein kinase B, gamma; protein kinase B-alpha; proto-oncogene c-AKT; proto-oncogene product; RAC protein kinase alpha RAC-PK alpha; RAC protein kinase beta RAC-PK beta; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; tumor rejection antigen peptide pRLib; unnamed protein product; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

          View more View less

          Gene Aliases: 2410016A19Rik; Akt; D930002M15Rik; LTR-akt; Nmf350; PKB; PKB/Akt; PKBalpha; PKBbeta; Pkbg; Rac

          View more View less

          UniProt ID: (Rat) P47196, (Mouse) P31750, (Mouse) Q60823, (Rat) P47197, (Mouse) Q9WUA6, (Rat) Q63484

          View more View less

          Entrez Gene ID: (Rat) 24185, (Mouse) 11651, (Mouse) 11652, (Rat) 25233, (Mouse) 23797, (Rat) 29414

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity GTPase activating protein binding identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity protein serine/threonine kinase binding TORC2 complex binding molecular function activator activity
          Process(es)
          positive regulation of protein phosphorylation glucose metabolic process protein phosphorylation insulin receptor signaling pathway positive regulation of signal transduction positive regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of sodium ion transport positive regulation of glucose metabolic process positive regulation of cell migration positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance response to insulin cellular response to insulin stimulus negative regulation of RNA splicing intracellular signal transduction negative regulation of apoptotic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import protein stabilization positive regulation of positive chemotaxis cellular response to high light intensity protein localization to plasma membrane positive regulation of protein targeting to membrane activation of GTPase activity retinal rod cell apoptotic process positive regulation of cap-dependent translational initiation negative regulation of PERK-mediated unfolded protein response positive regulation of cell motility osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis complement receptor mediated signaling pathway response to ischemia sphingosine-1-phosphate signaling pathway glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process translation activation-induced cell death of T cells inflammatory response cellular response to DNA damage stimulus response to oxidative stress epidermal growth factor receptor signaling pathway smoothened signaling pathway germ cell development apoptotic mitochondrial changes response to heat response to hormone gene expression negative regulation of autophagy positive regulation of endothelial cell migration negative regulation of gene expression positive regulation of fibroblast migration positive regulation of mitochondrial membrane potential regulation of neuron projection development response to muscle activity protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development lateral motor column neuron differentiation oogenesis stage cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration negative regulation of protein ubiquitination regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of protein localization negative regulation of superoxide anion generation positive regulation of peptidyl-serine phosphorylation response to fluid shear stress cellular response to heat interleukin-18-mediated signaling pathway cellular response to vascular endothelial growth factor stimulus cellular response to platelet-derived growth factor stimulus cellular response to decreased oxygen levels positive regulation of protein import into nucleus glucose homeostasis regulation of apoptotic process positive regulation of apoptotic process positive regulation of fat cell differentiation negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of organ growth protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation striated muscle cell differentiation positive regulation of protein metabolic process positive regulation of protein kinase B signaling response to growth hormone glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A protein localization to mitochondrion response to growth factor cellular response to mechanical stimulus cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia cellular hyperosmotic salinity response maintenance of protein location in mitochondrion cellular response to sorbitol negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis regulation of postsynapse organization regulation of tRNA methylation negative regulation of calcium import into the mitochondrion cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus cellular response to peptide negative regulation of cilium assembly negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway regulation of aerobic respiration positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface negative regulation of lymphocyte migration negative regulation of intrinsic apoptotic signaling pathway cytoplasmic translation gluconeogenesis translational initiation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport cytoskeleton organization cellular response to starvation phosphorylation cell migration negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process cellular response to nutrient levels negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation protein K29-linked ubiquitination SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling Golgi to plasma membrane protein transport proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cholesterol biosynthetic process positive regulation of cilium assembly negative regulation of translational initiation positive regulation of translational initiation protein maturation regulation of vesicle-mediated transport protein localization to cilium protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to calcium ion tricarboxylic acid metabolic process protein K6-linked ubiquitination tRNA (guanine-N7)-methylation cGAS/STING signaling pathway negative regulation of TORC1 signaling signal transduction regulation of mitochondrion organization positive regulation of TOR signaling positive regulation of angiogenesis positive regulation of cell size brain morphogenesis homeostasis of number of cells within a tissue positive regulation of cell migration involved in sprouting angiogenesis positive regulation of vascular endothelial cell proliferation positive regulation of artery morphogenesis negative regulation of cellular senescence response to hypoxia cellular response to hormone stimulus positive regulation of adipose tissue development
          It has to be done as per old AB suggested Products section.

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