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CD18 Monoclonal Antibody (IVA35), Alexa Fluor™ 488

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CD18 Monoclonal Antibody (IVA35), Alexa Fluor™ 488

Product Details

MA5-44128

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

Assay-dependent
-

Flow Cytometry (Flow)

2-5 µg/mL
-
Product Specifications

Species Reactivity

Bovine, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

IVA35

Immunogen

Bovine leukocytes
3D Epitope / Immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Size-exclusion chromatography

Storage buffer

PBS

Contains

15mM sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2913060

Product Specific Information

This antibody reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.

Target Information

CD18, also known as the integrin beta 2 subunit, is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. It heterodimerizes with the alpha chains of CD11a, CD11b, CD11c, and CD11d to form various leukocyte (beta2) integrins, which are crucial for cellular adhesion and signaling. The CD18/CD11a complex, known as lymphocyte function-associated antigen-1 (LFA-1), plays a significant role in cellular adhesion and inflammatory reactions. CD18 also forms heterodimers with CD11b (Mac-1, CR3), CD11c, and CD11d, contributing to the processes of cell adhesion and cell-surface mediated signaling. These integrins are essential for proper leukocyte migration and mediating intercellular contacts. The absence of CD18 leads to leukocyte adhesion deficiency type I (LAD1), a condition characterized by impaired leukocyte migration. Severe reduction in CD18 expression can result in the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, which can mediate leukotoxin-induced cytolysis. Defects in the CD18 gene are the cause of LAD1, and two transcript variants encoding the CD18 protein have been identified. The integrin-mediated responses are often a composite of the functions of its individual subunits, highlighting the importance of CD18 in immune function and disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: CD18 subunit; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; integrin beta chain, beta 2; integrin, beta 2 (antigen CD18 subunit (p95), lymphocyte function-associated antigen 1, integrin B2); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); Leu-CAM receptor; leukocyte adhesion protein beta-subunit precursor; leukocyte cell adhesion molecule CD18; leukocyte surface protein; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; putative; unnamed protein product

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Gene Aliases: CD18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7

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UniProt ID: (Bovine) P32592, (Human) P05107

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Entrez Gene ID: (Bovine) 281877, (Human) 3689

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Function(s)
protein binding signaling receptor activity metal ion binding beta-amyloid binding complement component C3b binding obsolete cell adhesion receptor activity integrin binding protein kinase binding ICAM-3 receptor activity heat shock protein binding cargo receptor activity macromolecular complex binding cell adhesion molecule binding integrin
Process(es)
phagocytosis receptor internalization cellular response to low-density lipoprotein particle stimulus positive regulation of leukocyte adhesion to vascular endothelial cell microglial cell activation leukocyte migration involved in inflammatory response receptor-mediated endocytosis phagocytosis, engulfment apoptotic process inflammatory response cell adhesion neuron cell-cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway I-kappaB kinase/NF-kappaB signaling cell-cell signaling regulation of cell shape neutrophil chemotaxis positive regulation of superoxide anion generation cell adhesion mediated by integrin cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion endodermal cell differentiation receptor clustering positive regulation of neutrophil degranulation endothelial cell migration cellular extravasation positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis negative regulation of dopamine metabolic process anatomical structure formation involved in morphogenesis regulation of peptidyl-tyrosine phosphorylation leukocyte adhesion to vascular endothelial cell positive regulation of protein targeting to membrane beta-amyloid clearance cell-cell adhesion neutrophil migration
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