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Invitrogen
GAPDH Recombinant Rabbit Monoclonal Antibody (BLR103H)
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FIGURE: 1 / 2
GAPDH Antibody (MA5-44397) in ICC/IF
Immunofluorescence analysis of GAPDH was performed using 70-80% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 45 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GAPDH Recombinant Rabbit Monoclonal Antibody (BLR103H) (Product # MA5-44397, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Pl... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-44397
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR103H
Immunogen
Peptide representing a region between residue 225 and 275 of Human glyceraldehyde-3-phosphate dehydrogenase.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
30 µg/mL
Amount
3 µg
Purification
purified
Storage buffer
BBS, pH 8.2, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AAG9; aging-associated gene 9 protein; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPA; GAPA-1; GAPC; GAPC-1; GAPC1; GAPCP-1; GAPCp1; GAPD; GAPDH; GAPDH-2; GAPDH-A; glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12); glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12); HGNC:4141; MGC88685; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; peptidyl-cysteine S-nitrosylase GAPDH; similar to Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA with GenBank Accession Number M33197.1; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product
Gene Aliases: G3PD; GAPD; Gapd-s; GAPD2; GAPDH-2; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35
UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Mouse) Q64467
Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Mouse) 14447
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
protein binding
microtubule binding
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
aspartic-type endopeptidase inhibitor activity
peptidyl-cysteine S-nitrosylase activity
identical protein binding
NADP binding
NAD binding
disordered domain specific binding
enzyme binding
glucose metabolic process
glycolytic process
sperm motility
positive regulation of glycolytic process
microtubule cytoskeleton organization
gluconeogenesis
regulation of macroautophagy
negative regulation of translation
positive regulation of type I interferon production
peptidyl-cysteine S-trans-nitrosylation
positive regulation of I-kappaB kinase/NF-kappaB signaling
protein stabilization
neuron apoptotic process
canonical glycolysis
antifungal innate immune response
antimicrobial humoral immune response mediated by antimicrobial peptide
cellular response to interferon-gamma
negative regulation of formation of translation preinitiation complex
positive regulation of cytokine production
negative regulation of endopeptidase activity
killing of cells of other organism
glyceraldehyde-3-phosphate biosynthetic process
defense response to fungus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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