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Invitrogen
5-Carboxylcytosine Recombinant Rabbit Monoclonal Antibody (RM462)
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FIGURE: 1 / 2
5-Carboxylcytosine Antibody (MA5-44548) in ELISA
ELISA analysis of DNA using 5-Carboxylcytosine Recombinant Monoclonal antibody (Product # MA5-44548) at a dilution of 1 µg/mL. The plate was coated with different concentrations of linear ssDNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-44548
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM462
Immunogen
KLH-conjugated 5-Carboxylcytosine.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts to human NeuN (Fox3, RBFOX3). The clone may also react to mouse or rat NeuN, as predicted by immunogen homology.
Target Information
5-carboxylcytosine is the final oxidized derivative of 5-methylcytosine, a type of DNA methylation. 5-methylcytosine is oxidized to 5-hydroxymethylcytosine which is then oxidized to 5-formylcytosine and later to 5-carboxylcytosine. These oxidations are catalyzed by the Ten-Eleven Translocation (TET) enzyme family. 5-carboxylcytosine has been shown to cause reduced fidelity of RNA Polymerase II. Decreased levels of 5-hydroxymethylcytosine has been found in some cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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