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Invitrogen
SOS1 Recombinant Rabbit Monoclonal Antibody (JE30-06)
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FIGURE: 1 / 1
SOS1 Antibody (MA5-44843) in WB
Western blot analysis of SOS1 in different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST 1 hour at room temperature. Samples were incubated in SOS1 Monoclonal antibody (Product # MA5-44843) using a dilution of 1:500 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate; Lane 2: Hela cell lysate; Lane 3: Mouse hippocampus tissue lysate; Lane 4: Rat ovary tis... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-44843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE30-06
Immunogen
Recombinant protein within human SOS1 aa 1-150/1,333.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Son of sevenless (SOS) is a guanine nucleotide exchange factor that activates Ras in response to growth factor stimulation. Sos1, and its closely related Sos2 paralog, are comprised of an N-terminal histone-binding domain, a RacGEF catalytic (DH) domain juxtaposed with a PH domain, a Ras exchanger motif (REM) domain, a RasGEF catalytic domain, and a C-terminal proline-rich region. Sos1 catalyzes the GDP-GTP exchange within the membrane-bound GTPase Ras and thereby switches on a key signaling circuit that involves the activation of MAPK cascade central to cellular proliferation, survival and differentiation. Growth factor stimulation rapidly induces the phosphorylation of Sos on multiple serine and threonine sites. Phosphorylation of residues within the Sos C-terminus by the MAP/ERK kinase disrupts the Grb2-Sos complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G GINGF; gingival fibromatosis; gingival fibromatosis, hereditary, 1; guanine nucleotide exchange factor; guanine nucleotide releasing factor; ras guanine exchange factor; son-of-sevenless 1; unnamed protein product
Gene Aliases: 4430401P03Rik; 9630010N06; GF1; GGF1; GINGF; HGF; NS4; SOS-1
UniProt ID: (Human) Q07889, (Mouse) Q62245
Entrez Gene ID: (Human) 6654, (Rat) 313845, (Mouse) 20662
response to ischemia
regulation of transcription from RNA polymerase II promoter
signal transduction
epidermal growth factor receptor signaling pathway
small GTPase mediated signal transduction
Ras protein signal transduction
axon guidance
insulin receptor signaling pathway
Schwann cell development
cytokine-mediated signaling pathway
positive regulation of Rac protein signal transduction
Fc-epsilon receptor signaling pathway
T cell activation
regulation of cell proliferation
myelination
positive regulation of epidermal growth factor receptor signaling pathway
insulin-like growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
B cell receptor signaling pathway
leukocyte migration
positive regulation of small GTPase mediated signal transduction
B cell homeostasis
hair follicle development
lymphocyte homeostasis
heart morphogenesis
cardiac atrium morphogenesis
pericardium morphogenesis
vitellogenesis
fibroblast growth factor receptor signaling pathway
regulation of T cell differentiation in thymus
multicellular organism growth
regulation of T cell proliferation
blood vessel morphogenesis
palate development
eyelid development in camera-type eye
heart trabecula morphogenesis
midbrain morphogenesis
regulation of pro-B cell differentiation
ERBB2-ERBB3 signaling pathway
positive regulation of Ras protein signal transduction
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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