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Invitrogen
CaMKII alpha Monoclonal Antibody (6G9), FITC
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FIGURE: 1 / 4
CaMKII alpha Antibody (MA5-45144) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol for 10 minutes at -20°C. Samples were incubated with CaMKII monoclonal antibody (Product # MA5-45144) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45144
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6G9
Immunogen
Partially purified rat CaMKII
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.1 µg/mL was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.|Detects approximately 50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha CaM kinase II; alpha-CaMKII; alpha-KAP; Ca2+/calmodulin-dependent protein kinase II alpha; Calci; calcium-calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha; calcium/calmodulin-dependent protein kinase type II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; EC 2.7.11.17; OTTHUMP00000165787; OTTHUMP00000165788; Phospho-CaMKI; unnamed protein product
Gene Aliases: CAMKA; CaMKII; CaMKIIalpha; CaMKIINalpha; mKIAA0968; MRD53; MRT63; PK2CDD; PKCCD
UniProt ID: (Human) Q9UQM7, (Mouse) P11798, (Rat) P11275
Entrez Gene ID: (Bovine) 530719, (Human) 815, (Mouse) 12322, (Rat) 25400
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
glutamate receptor binding
identical protein binding
protein homodimerization activity
protein serine kinase activity
kinase activity
GTPase activating protein binding
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
response to ischemia
calcium ion transport
positive regulation of cardiac muscle cell apoptotic process
cellular response to interferon-beta
angiotensin-activated signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of JAK-STAT cascade
regulation of neurotransmitter secretion
regulation of neuronal synaptic plasticity
positive regulation of calcium ion transport
long-term synaptic potentiation
dendritic spine development
cellular response to interferon-gamma
negative regulation of ferroptosis
regulation of mitochondrial membrane permeability involved in apoptotic process
regulation of protein localization to plasma membrane
regulation of endocannabinoid signaling pathway
regulation of neuron migration
protein phosphorylation
JAK-STAT cascade
peptidyl-serine phosphorylation
ionotropic glutamate receptor signaling pathway
protein autophosphorylation
dendrite morphogenesis
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
neurotransmitter receptor transport to plasma membrane
peptidyl-threonine autophosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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