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Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (HL127)

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Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (HL127)

Product Details

MA5-46892

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

HL127

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr576 of human FAK. The exact sequence is proprietary.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2937964

Product Specific Information

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK; FAK-Del33; exon 33 deletion splicing mutant; FAK-related non-kinase polypeptide; focal adhesion kinase-related nonkinase; focal ashension kinase 1; non-receptor protein tyrosine kinase; pp125 PTK2; pp125FAK; protein phosphatase 1 regulatory subunit 71; protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; unnamed protein product

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Gene Aliases: FADK; FADK 1; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71

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UniProt ID: (Human) Q05397, (Rat) O35346, (Mouse) P34152

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Entrez Gene ID: (Human) 5747, (Rat) 25614, (Mouse) 14083

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Function(s)
actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein tyrosine phosphatase activity integrin binding protein binding ATP binding JUN kinase binding protein kinase binding protein phosphatase binding SH2 domain binding molecular function activator activity phosphatase binding identical protein binding phosphatidylinositol 3-kinase binding macromolecular complex binding
Process(es)
MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration nuclear migration signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade positive regulation of cell proliferation regulation of cell shape response to xenobiotic stimulus response to mechanical stimulus response to glucose negative regulation of autophagy positive regulation of cardiac muscle hypertrophy regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition positive regulation of macrophage chemotaxis positive regulation of fibroblast migration positive regulation of smooth muscle cell migration cell migration peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion cell differentiation regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis response to estradiol negative regulation of cell adhesion mediated by integrin positive regulation of cell-cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation regulation of cell proliferation vasodilation negative regulation of apoptotic process endothelial cell migration fat cell differentiation regulation of osteoblast differentiation positive regulation of cell adhesion negative regulation of organ growth response to arsenic-containing substance protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis regulation of neurogenesis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to morphine cellular response to transforming growth factor beta stimulus positive regulation of wound healing positive regulation of macrophage proliferation regulation of substrate adhesion-dependent cell spreading regulation of modification of postsynaptic actin cytoskeleton positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis regulation of cell adhesion mediated by integrin regulation of growth placenta development regulation of cell-matrix adhesion heart morphogenesis axon guidance regulation of endothelial cell migration establishment of cell polarity detection of muscle stretch netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of cell differentiation vascular endothelial growth factor receptor signaling pathway cell motility regulation of multicellular organismal process regulation of cytoskeleton organization positive regulation of focal adhesion assembly vascular endothelial cell response to oscillatory fluid shear stress
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