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Invitrogen
NF-H Monoclonal Antibody (AH1)
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FIGURE: 1 / 2
NF-H Antibody (MA5-47374) in IHC (PFA)
Immunohistochemistry analysis of NF-H in rat cerebellum section. Samples were stained with NF-H monoclonal antibody (Product # MA5-47473) using a dilution of 1:2,000 (green). Costained with rabbit pAb to FOX3/NeuN (Product # PA5-143605) dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections. The NF-H ant... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-47374
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
AH1
Immunogen
Native NF-H purified from bovine spinal cord
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Protein A/G
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; heavy neurofilament polypeptide; heavy neurofilament subunit; high molecular weight neurofilament; hSTEp; NEF3; neurofilament; neurofilament 200kDa; neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; Nfh; NF200; NHC; unnamed protein product
Gene Aliases: CMT2CC; mKIAA0845; NF-H; NF200; NFH
UniProt ID: (Human) P12036, (Mouse) P19246
Entrez Gene ID: (Horse) 100064328, (Human) 4744, (Pig) 100156492, (Mouse) 380684, (Rat) 24587
microtubule cytoskeleton organization
ovarian follicle atresia
axonogenesis
axon regeneration
neurofilament bundle assembly
intermediate filament cytoskeleton organization
intermediate filament bundle assembly
peripheral nervous system neuron axonogenesis
neurofilament cytoskeleton organization
axon development
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
regulation of organelle transport along microtubule
response to sodium arsenite
cellular response to leukemia inhibitory factor
cell projection assembly
spinal cord development
hippocampus development
cerebral cortex development
neuron projection development
cellular response to oxidative stress
response to cocaine
cellular response to estradiol stimulus
cellular response to hydroperoxide
response to acrylamide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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