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CD16/CD32 Chimeric Recombinant Rabbit Monoclonal Antibody (2.4G2)

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CD16/CD32 Chimeric Recombinant Rabbit Monoclonal Antibody (2.4G2)

Product Details

MA5-47770

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

Flow Cytometry (Flow)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Inhibition Assays (Inhibition)

Assay-dependent
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2.4G2

Immunogen

Mouse BALB/c Macrophage J774 Cell Line.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

200 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074220

Product Specific Information

Specificity: This antibody binds to an epitope on the extracellular domains of the mouse Fc-gamma-III and Fc-gamma-II, thereby inhibiting their ability to bind antibodies via their Fc region receptors. Also binding the Fc-gamma-I receptor (CD64) via its Fc domain.

This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD16A; CD16B; CD32 receptor 2; CDw32; fc gamma receptor IIB; Fc gamma receptor III; Fc gamma receptor precursor; Fc gamma RIIB; fc-gamma RII; Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma-1/gamma-2bR receptor precursor; Fc-gamma-R-alpha receptor; Fc-gamma-R-beta-1 precursor; Fc-gamma-R-beta-2 precursor; Fc-gamma-RII; fc-gamma-RIIB; Fc-gamma-RIIc; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; igG Fc receptor II beta; igG Fc receptor III; low affinity immunoglobulin gamma Fc region receptor II; lymphocyte antigen 17; RP11-5K23.1; unnamed protein product

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Gene Aliases: CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

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Function(s)
beta-amyloid binding protein binding IgG receptor activity low-affinity IgG receptor activity IgG binding immunoglobulin binding macromolecular complex binding immunoglobulin receptor binding
Process(es)
antibody-dependent cellular cytotoxicity negative regulation of type I hypersensitivity immune complex clearance negative regulation of immunoglobulin production negative regulation of inflammatory response to antigenic stimulus negative regulation of acute inflammatory response to antigenic stimulus negative regulation of humoral immune response mediated by circulating immunoglobulin receptor-mediated endocytosis phagocytosis, engulfment defense response cell surface receptor signaling pathway response to bacterium immunoglobulin mediated immune response dendrite development antigen processing and presentation of exogenous peptide antigen via MHC class II cerebellum development negative regulation of B cell proliferation endocytic recycling negative regulation of interleukin-10 production positive regulation of tumor necrosis factor production negative regulation of neutrophil apoptotic process Fc-gamma receptor signaling pathway positive regulation of JNK cascade regulation of long-term neuronal synaptic plasticity negative regulation of phagocytosis positive regulation of phagocytosis regulation of immune response negative regulation of immune response cellular response to molecule of bacterial origin Fc receptor-mediated immune complex endocytosis regulation of dendritic spine maintenance regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway cellular response to beta-amyloid positive regulation of response to endoplasmic reticulum stress positive regulation of type IIa hypersensitivity positive regulation of type III hypersensitivity positive regulation of type I hypersensitivity serotonin secretion phagocytosis, recognition neutrophil chemotaxis antigen processing and presentation of exogenous peptide antigen via MHC class I mast cell activation
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