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Invitrogen
VISTA Recombinant Rabbit Monoclonal Antibody (PSH0-64)
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FIGURE: 1 / 7
VISTA Antibody (MA5-49363) in IHC (P)
Immunohistochemical analysis of VISTA on formalin-fixed paraffin-embedded human placenta tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with VISTA Rabbit Recombinant Monoclonal Antibody (PSH0-64) (Product # MA5-49363) at 1:1,000 dilution for 1 hour at roo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-49363
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-64
Immunogen
Recombinant protein within human vista aa 1-194 / 311.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Sequence Similarities: 95% Mouse/Rat.
Tissue Specificity: Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b-positive neutrophils, at low levels on CD4-positive and CD8-positive T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta, spleen, plasma blood leukocytes, and lung. Expressed at moderate levels in lymph node, bone marrow, fat, uterus, and trachea. Has low expression levels in other tissues.
Positive Control: Human brain tissue, human placenta tissue, human tonsil tissue, A375 cell lysate, HeLa cell lysate, human lung tissue lysate.
Subcellular Location: Cell membrane.
Predicted band size: 34 kDa.
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Death Domain1alpha; Gi24; PD-H1; PDCD1 homolog; platelet receptor GI24; sisp-1; stress-induced secreted protein-1; V-domain Ig suppressor of T cell activation; V-set domain-containing immunoregulatory receptor
Gene Aliases: B7-H5; B7H5; C10orf54; DD1alpha; Dies1; GI24; PD-1H; PP2135; SISP1; VISTA
UniProt ID: (Human) Q9H7M9
Entrez Gene ID: (Human) 64115
positive regulation of gene expression
positive regulation of endopeptidase activity
positive regulation of cell migration
zymogen activation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
negative regulation of interleukin-17 production
negative regulation of tumor necrosis factor production
positive regulation of regulatory T cell differentiation
negative regulation of alpha-beta T cell activation
regulation of immune response
positive regulation of collagen catabolic process
negative regulation of CD4-positive, alpha-beta T cell proliferation
negative regulation of CD8-positive, alpha-beta T cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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