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Invitrogen
NDUFC2 Recombinant Rabbit Monoclonal Antibody (JE65-31)
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FIGURE: 1 / 1
NDUFC2 Antibody (MA5-50672) in WB
Western blot analysis of NDUFC2 in different various lysates. Lane 1: 293T cell lysate; Lane 2: Hela cell lysate; Lane 3: A549 cell lysate; Lane 4: Human kidney tissue lysate (20 µg/Lane)Lysates/proteins at 10 µg/Lane. Predicted band size: 14 kDa. Observed band size: 14 kDa. Exposure time: 1 minute;15% SDS-PAGE gel. Primary antibody NDUFC2 recombinant monoclonal antibody (Product # MA5-50672) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:3... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-50672
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE65-31
Immunogen
Recombinant protein within human NDUFC2 aa 1-119.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: 293T cell lysate, Hela cell lysate, A549 cell lysate, human kidney tissue lysate.
Predicted band size: 14 kDa
Subcellular Location: Mitochondrion inner membrane.
Target Information
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-49kD; CI-B145b; complex 1, mitochondrial respiratory chain, 49-KD subunit; complex I 49kDa subunit; complex I iron sulphur protein subunit; 49kDa subunit; complex I subunit; complex I subunit B14.5b; complex I-B14.5b; Complex I-B145b; HLC-1; human lung cancer oncogene 1 protein; lung cancer oncogene 2; NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase); NADH-ubiquinone oxidoreductase 49 kDa subunit; NADH-ubiquinone oxidoreductase NDUFS2 subunit; NADH-ubiquinone oxidoreductase subunit B14.5b; NADHDH2; NDUFC2; unnamed protein product
Gene Aliases: B14.5b; CI-49; CI-B14.5b; HLC-1; LHONAR2; MC1DN36; MC1DN6; NADHDH2
UniProt ID: (Human) O95298, (Human) O75306
Entrez Gene ID: (Human) 4718, (Human) 4720
mitochondrial electron transport, NADH to ubiquinone
aerobic respiration
positive regulation of mitochondrial membrane potential
mitochondrial respiratory chain complex I assembly
mitochondrial ATP synthesis coupled proton transport
negative regulation of NIK/NF-kappaB signaling
hydrogen ion transmembrane transport
negative regulation of reactive oxygen species biosynthetic process
positive regulation of ATP biosynthetic process
neurogenesis
gliogenesis
mitochondrial ATP synthesis coupled electron transport
neural precursor cell proliferation
cellular response to oxygen levels
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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