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Invitrogen
RAP1A/RAP1B Recombinant Rabbit Monoclonal Antibody (JE35-61)
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FIGURE: 1 / 2
RAP1A/RAP1B Antibody (MA5-50823) in WB
Western blot analysis of RAP1A/RAP1B in different various lysates. Lane 1: HeLa cell lysate; Lane 2: Jurkat cell lysate; Lane 3: NIH/3T3 cell lysate; Lane 4: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa. Observed band size: 20 kDa. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Primary antibody RAP1A/RAP1B recombinant monoclonal antibody (Product # MA5-50823) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-50823
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE35-61
Immunogen
Synthetic peptide within Human RAP1A aa 93-142/184.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, HEK-293 cell lysate, HEK-293.
Predicted band size: 20 kDa
Subcellular Location: Cell membrane, Cytoplasm, Cytoplasm, perinuclear region, Cell junction, Early endosome; Cell membrane, Cytoplasm, cytosol, Cell junction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTP-binding protein smg p21A; GTP-binding protein smg p21B; GTP-binding protein SMG-P21A; NB4 apoptosis/differentiation related protein; RAP1B; Ras family small GTP binding protein RAP1B; Ras-like GTPase; RAS-related protein 1a; Ras-related protein Krev-1; RAS-related protein RAP1B; small GTP binding protein; unnamed protein product
Gene Aliases: 2810443E11Rik; C21KG; G-22K; K-REV; KREV-1; KREV1; RAL1B; RAP1; SMGP21; THC11
UniProt ID: (Human) P62834, (Human) P61224, (Mouse) P62835, (Mouse) Q99JI6
Entrez Gene ID: (Human) 5906, (Human) 5908, (Mouse) 109905, (Mouse) 215449
signal transduction
adenylate cyclase-activating G-protein coupled receptor signaling pathway
Rap protein signal transduction
positive regulation of integrin activation
establishment of endothelial barrier
cellular response to cAMP
modification of postsynaptic structure
regulation of cell junction assembly
regulation of establishment of cell polarity
negative regulation of synaptic vesicle exocytosis
small GTPase mediated signal transduction
response to carbohydrate
positive regulation of neuron projection development
negative regulation of collagen biosynthetic process
nerve growth factor signaling pathway
positive regulation of GTPase activity
positive regulation of protein kinase activity
positive regulation of glucose import
positive regulation of phagocytosis
positive regulation of Fc receptor mediated stimulatory signaling pathway
positive regulation of ERK1 and ERK2 cascade
protein localization to plasma membrane
response to antineoplastic agent
liver regeneration
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
cellular response to forskolin
cellular response to nerve growth factor stimulus
positive regulation of vasculogenesis
cell proliferation
negative regulation of calcium ion-dependent exocytosis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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