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Invitrogen
HDGF Recombinant Mouse Monoclonal Antibody (H3)
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FIGURE: 1 / 3
HDGF Antibody (MA5-51868) in ICC/IF
Immunocytochemistry analysis of HDGF in paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton using HDGF Recombinant Mouse Monoclocal Antibody (Product # MA5-51868) at a 1:100 dilution for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: primary antibody, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Product # MA5-47823) followed by staining with Alex... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-51868
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
H3
Immunogen
The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody C1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the HDGF protein.
Target Information
HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686J1764; epididymis secretory sperm binding protein; FLJ96580; hepatoma derived growth factor; high mobility group protein 1-like 2; unnamed protein product
Gene Aliases: D3Ertd299e; HMG1L2
UniProt ID: (Human) P51858, (Mouse) P51859
Entrez Gene ID: (Human) 3068, (Mouse) 15191
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription corepressor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription corepressor activity
RNA binding
actin binding
protein binding
heparin binding
tubulin binding
growth factor activity
negative regulation of transcription from RNA polymerase II promoter
chromatin remodeling
signal transduction
protein localization to nucleus
positive regulation of transcription from RNA polymerase II promoter
positive regulation of cell division
negative regulation of neuron apoptotic process
cellular response to interleukin-7
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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