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Invitrogen
CRYAB Recombinant Rabbit Monoclonal Antibody (23GB3500)
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FIGURE: 1 / 3
CRYAB Antibody (MA5-52556) in ICC/IF
Immunocytochemistry analysis of CRYAB in HT-1080 cells. Nuclei were stained blue with DAPI; CRYAB recombinant monoclonal antibody (Product # MA5-52556) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-52556
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB3500
Immunogen
A synthetic peptide of human CRYAB (124-175AA).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.4 mg/mL
Amount
40 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha(B)-crystallin; CryAB antibody; Crystallin, alpha polypeptide 2; epididymis secretory protein Li 101; heat shock 20 kDa protein-like protein; heat shock protein beta-5; heat shock protein beta-5; heat shock protein family B member 5; heat-shock 20 kD like-protein; ORF1; ORF2; ORF32; renal carcinoma antigen NY-REN-27; Rosenthal fiber component; similar to human alpha B-crystallin Rosenthal fiber component encoded by GenBank Accession Number S45630; unnamed protein product
Gene Aliases: AACRYA; CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; MFM2A; MFM2B
UniProt ID: (Human) P02511, (Rat) P23928
Entrez Gene ID: (Human) 1410, (Rat) 25420
response to hypoxia
lens development in camera-type eye
protein folding
glutathione metabolic process
tubulin complex assembly
muscle organ development
response to heat
negative regulation of gene expression
negative regulation of cell growth
microtubule polymerization or depolymerization
negative regulation of protein complex assembly
response to estradiol
negative regulation of intracellular transport
protein refolding
response to hydrogen peroxide
camera-type eye development
negative regulation of apoptotic process
regulation of programmed cell death
negative regulation of transcription, DNA-templated
protein stabilization
stress-activated MAPK cascade
apoptotic process involved in morphogenesis
cellular response to gamma radiation
negative regulation of amyloid fibril formation
negative regulation of reactive oxygen species metabolic process
muscle contraction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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