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Invitrogen
Nicastrin Recombinant Rabbit Monoclonal Antibody (23GB1040)
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FIGURE: 1 / 4
Nicastrin Antibody (MA5-53744) in ICC/IF
Immunocytochemistry analysis of Nicastrin in HepG2 cells. Nuclei were stained blue with DAPI; Nicastrin recombinant monoclonal antibody (Product # MA5-53744) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-53744
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB1040
Immunogen
A synthetic peptide of human Nicastrin (696-709AA).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anterior pharynx-defective 2; KIAA0253; OTTHUMP00000031840; type 1 transmembrane protein; type I transmembrane protein; unnamed protein product
Gene Aliases: 9430068N19Rik; Aph2; ATAG1874; D1Dau13e; Kiaa0253; mKIAA0253; Nct
UniProt ID: (Human) Q92542, (Rat) Q8CGU6, (Mouse) P57716
Entrez Gene ID: (Human) 23385, (Rat) 289231, (Mouse) 59287
myeloid cell homeostasis
membrane protein ectodomain proteolysis
dopamine receptor signaling pathway
glutamate receptor signaling pathway
Notch receptor processing
learning or memory
protein processing
cerebellum development
central nervous system myelination
adult behavior
membrane protein intracellular domain proteolysis
beta-amyloid formation
T cell proliferation
amyloid precursor protein metabolic process
amyloid precursor protein biosynthetic process
positive regulation of amyloid precursor protein biosynthetic process
amyloid precursor protein catabolic process
beta-amyloid metabolic process
epithelial cell proliferation
neuron apoptotic process
cellular response to calcium ion
regulation of long-term synaptic potentiation
short-term synaptic potentiation
proteolysis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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