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Invitrogen

pro-Caspase 3 Recombinant Rabbit Monoclonal Antibody (23GB715)

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pro-Caspase 3 Recombinant Rabbit Monoclonal Antibody (23GB715)

Product Details

MA5-54082

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Flow Cytometry (Flow)

1:200-1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

23GB715

Immunogen

A synthetic peptide of human pro Caspase 3 (1-28AA).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Amount

70 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3248553

Target Information

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; CPP-32; cysteine protease CPP32; PARP cleavage protease; procaspase3; protein Yama; SREBP cleavage activity 1; unnamed protein product

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Gene Aliases: CPP32; CPP32B; SCA-1

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UniProt ID: (Human) P42574

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Entrez Gene ID: (Human) 836

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Function(s)
protease binding endopeptidase activity aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding enzyme activator activity peptidase activity cysteine-type peptidase activity phospholipase A2 activator activity macromolecular complex binding
Process(es)
luteolysis response to hypoxia negative regulation of cytokine production proteolysis apoptotic process axonal fasciculation learning or memory intrinsic apoptotic signaling pathway in response to osmotic stress response to xenobiotic stimulus response to glucose response to metal ion response to X-ray regulation of macroautophagy protein processing hippocampus development protein catabolic process neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation regulation of protein stability response to cobalt ion response to estradiol response to lipopolysaccharide regulation of protein localization glial cell apoptotic process response to tumor necrosis factor response to nicotine intracellular signal transduction swimming behavior response to hydrogen peroxide positive regulation of apoptotic process response to amino acid regulation of neuron apoptotic process positive regulation of neuron apoptotic process response to ethanol neurotrophin TRK receptor signaling pathway striated muscle cell differentiation response to glucocorticoid protein maturation pyroptosis leukocyte apoptotic process response to anesthetic cellular response to staurosporine apoptotic signaling pathway intrinsic apoptotic signaling pathway execution phase of apoptosis regulation of synaptic vesicle cycle synapse pruning positive regulation of pyroptotic inflammatory response positive regulation of beta-amyloid formation positive regulation of apoptotic DNA fragmentation response to insulin-like growth factor stimulus
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