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          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          Phospho-SRC (Tyr416) Recombinant Rabbit Monoclonal Antibody (8W3U0)

          View all (81) SRC antibodies

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          Cite Phospho-SRC (Tyr416) Recombinant Rabbit Monoclonal Antibody (8W3U0)

          Additional Information:
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          • Antibody Testing Data (3)
          Phospho-SRC (Tyr416) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-SRC (Tyr416) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Phospho-SRC (Tyr416) Antibody (MA5-55322) in WB

          Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SRC (Tyr416) Recombinant Monoclonal Antibody (Product # MA5-55322) at 1:300 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37&(8)451; for 30 minutes after serum-starvation overnight followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-SRC (Tyr416) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr416) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr416) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-SRC (Tyr416) Recombinant Rabbit Monoclonal Antibody (8W3U0)

          Product Details

          MA5-55322

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          8W3U0

          Immunogen

          Synthetic peptide
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2.10 mg/mL

          Amount

          210 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          RRID

          AB_3667563

          Product Specific Information

          Immunogen Sequence: NEYTAR

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Bioinformatics

          Protein Aliases: c-Src; EC 2.7.10.2; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-src; pp60src; proto-oncogene c-Src; Rous sarcoma oncogene; RP5-823N20.1; tyrosine protein kinase c-src; tyrosine protein kinase pp60-c-src; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

          View more View less

          UniProt ID: (Rat) Q9WUD9

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          Entrez Gene ID: (Rat) 83805

          View more View less

          Function(s)
          protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein kinase C binding receptor binding insulin receptor binding integrin binding protein binding ATP binding phospholipase activator activity kinase activity enzyme binding protein kinase binding protein domain specific binding heme binding estrogen receptor binding receptor activator activity ionotropic glutamate receptor binding SH2 domain binding phospholipase binding ion channel binding macromolecular complex binding ephrin receptor binding cell adhesion molecule binding phosphoprotein binding BMP receptor binding connexin binding scaffold protein binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter positive regulation of cytokine production protein phosphorylation cell adhesion epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway gamete generation spermatogenesis lactation learning or memory positive regulation of cell proliferation response to xenobiotic stimulus response to mechanical stimulus response to acidic pH positive regulation of gene expression negative regulation of gene expression regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of glucose metabolic process positive regulation of protein processing skeletal muscle cell proliferation positive regulation of smooth muscle cell migration cell migration peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion cell differentiation positive regulation of cell migration forebrain development negative regulation of protein complex assembly protein destabilization response to nutrient levels negative regulation of telomere maintenance cellular response to insulin stimulus regulation of intracellular estrogen receptor signaling pathway response to ATP regulation of toll-like receptor 3 signaling pathway adherens junction organization substrate adhesion-dependent cell spreading cellular response to reactive oxygen species positive regulation of Rac protein signal transduction positive regulation of dephosphorylation negative regulation of hippo signaling intracellular signal transduction osteoclast development cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation angiotensin-activated signaling pathway vasodilation odontogenesis response to hydrogen peroxide positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly positive regulation of MAP kinase activity transcytosis bone resorption positive regulation of Notch signaling pathway positive regulation of bone resorption positive regulation of cell adhesion positive regulation of cell size positive regulation of glycolytic process positive regulation of Ras protein signal transduction positive regulation of insulin receptor signaling pathway platelet-derived growth factor receptor signaling pathway neurotrophin TRK receptor signaling pathway focal adhesion assembly behavioral response to cocaine oogenesis positive regulation of peptidyl-tyrosine phosphorylation progesterone receptor signaling pathway positive regulation of small GTPase mediated signal transduction positive regulation of protein transport response to mineralocorticoid myoblast proliferation response to electrical stimulus negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly intestinal epithelial cell development interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade response to fatty acid response to interleukin-1 cellular response to lipopolysaccharide cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fatty acid cellular response to hypoxia cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly DNA biosynthetic process regulation of heart rate by cardiac conduction positive regulation of canonical Wnt signaling pathway positive regulation of wound healing cell-cell adhesion positive regulation of protein localization to nucleus positive regulation of intracellular signal transduction negative regulation of neutrophil activation positive regulation of cellular protein localization positive regulation of TORC1 signaling cellular response to angiotensin positive regulation of vascular smooth muscle cell proliferation positive regulation of outer hair cell apoptotic process cellular response to prolactin positive regulation of neural precursor cell proliferation positive regulation of male germ cell proliferation positive regulation of lamellipodium morphogenesis positive regulation of platelet-derived growth factor receptor-beta signaling pathway regulation of early endosome to late endosome transport negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway regulation of caveolin-mediated endocytosis
          It has to be done as per old AB suggested Products section.

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