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PSMB10 Recombinant Rabbit Monoclonal Antibody (3J3G5)
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FIGURE: 1 / 2
PSMB10 Antibody (MA5-55536) in WB
Western Blot analysis of various lysates using PSMB10 Recombinant Monoclonal Antibody (Product # MA5-55536) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-55536
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3J3G5
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
Immunogen Sequence: MLKPALEPRG GFSFENCQRN ASLERVLPGL KVPHARKTGT TIAGLVFQDG VILGADTRAT NDSVVADKSC EKIHFIAPKI YCCGAGVAAD AEMTTRMVAS KMELHALSTG REPRVATVTR ILRQTLFRYQ GHVGASLIVG GVDLTGPQLY GVHPHGSYSR LPFTALGSGQ DAALAVLEDR FQPNMTLEAA QGLLVEAVTA GILGDLGSGG NVDACVITKT GAKLLRTLSS PTEPVKRSGR YHFVPGTTAV LTQTVKPLTL ELVEETVQAM EVE
Target Information
The 20S proteasome is a protease complex that is responsible for cytosolic protein degradation and generation of peptide ligands for major histocompatibility complex (MHC) class I molecules, either in their final form or in the form of amino-terminally extended precursors. Upon IFN-gamma stimulation of cells, three constitutively expressed subunits of the 20S proteasome are replaced by inducible subunits LMP2 (low-molecular mass polypeptide 2), LMP7, and MECL-1 (multicatalytic endopeptidase complex-like-1, LMP10). LMP2, LMP7, and MECL-1 subunits form immunoproteasomes, which are associated with more efficient class I antigen processing and presentation. Independent assortment of LMP-2, LMP-7, and MECL-1 into different proteasome complexes can lead to 36 unique proteasome subsets, which may mediate differences in the cleavage specificities/cleavage motifs of proteins subject to constitutive- and immuno-proteasomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: low molecular mass protein 10; macropain subunit MECl-1; MGC1665; multicatalytic endopeptidase complex subunit MECl-1; OTTHUMP00000174858; prosome Mecl1; proteasome (prosome, macropain) subunit, beta type 10; proteasome (prosome, macropain) subunit, beta type, 10; proteasome (prosomome, macropain) subunit, beta type 10; proteasome catalytic subunit 2i; Proteasome MECl 1; proteasome MECl-1; proteasome subunit beta 10; proteasome subunit beta 7i; proteasome subunit beta-2i; proteasome subunit beta2i; proteasome subunit MECL1; PSMB 10; PSMB10; unnamed protein product
Gene Aliases: beta2i; IMD121; LMP10; Mecl-1; MECL1; PRAAS5
UniProt ID: (Human) P40306, (Mouse) O35955, (Rat) Q4KM35
Entrez Gene ID: (Human) 5699, (Mouse) 19171, (Rat) 291983
cell morphogenesis
protein catabolic process
T cell proliferation
proteasome-mediated ubiquitin-dependent protein catabolic process
immune system process
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
humoral immune response
proteasomal protein catabolic process
proteasomal ubiquitin-independent protein catabolic process
positive regulation of interferon-gamma production
positive regulation of interleukin-2 production
positive regulation of tumor necrosis factor production
response to interferon-gamma
CD8-positive, alpha-beta T cell differentiation
thymic T cell selection
T-helper 1 cell differentiation
negative regulation of regulatory T cell differentiation
T-helper 17 cell differentiation
CD8-positive, alpha-beta T cell homeostasis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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