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Invitrogen
UBE1L Recombinant Rabbit Monoclonal Antibody (6V3Y5)
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FIGURE: 1 / 1
UBE1L Antibody (MA5-55587) in WB
Western blot analysis of lysates from HEL cells, using UBE1L Recombinant Monoclonal Antibody (Product # MA5-55587) at 1:500 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-55587
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6V3Y5
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Ube1L UBA7.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
Immunogen Sequence: MDALDASKLL DEELYSRQLY VLGSPAMQRI QGARVLVSGL QGLGAEVAKN LVLMGVGSLT LHDPHPTCWS DLAAQFLLSE QDLERSRAEA SQELLAQLNR
Target Information
UBE1L (ubiquitin-like modifier-activating enzyme 7) activates ubiquitin by first adenylating with ATP at its C-terminal glycine residue and then linking that residue to the side chain of a cysteine residue in E1. This activation results in an ubiquitin-E1 thioester and free AMP. UBE1L also catalyzes the ISGylation of influenza A virus NS1 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC12713; similar to ubiquitin activating enzymes; UBA1, ubiquitin-activating enzyme E1 homolog B; UBA7, ubiquitin-activating enzyme E1; ubiquitin-activating enzyme 7; ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-related protein; ubiquitin-activating enzyme-2; unnamed protein product
Gene Aliases: 1300004C08Rik; D8; UBA1B; UBE1L; UBE2; UBE7
UniProt ID: (Human) P41226, (Mouse) Q9DBK7
Entrez Gene ID: (Human) 7318, (Rat) 301000, (Mouse) 74153
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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