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Invitrogen
Myogenin Recombinant Rabbit Monoclonal Antibody (JE60-39)
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FIGURE: 1 / 7
Myogenin Antibody (MA5-55604) in ICC/IF
Immunofluorescence analysis of Myogenin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myogenin Recombinant Rabbit Monoclonal Antibody (JE60-39) (Product # MA5-55604) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-55604
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE60-39
Immunogen
Synthetic peptide within human myogenin aa 1-50/224.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
Product Specific Information
Positive Control: Differentiated C2C12 cell lysate.
Tissue Specificity: Group enriched (skeletal muscle, tongue).
Subcellular Location: Nucleus.
Sequence Similarities: 100% Mouse/Rat.
Predicted band size: 25 kDa.
Target Information
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class C basic helix-loop-helix protein 3; MYOD1-related protein; myogenic factor 4; Myogenic factor-4; myogenic regulatory transcription factor; myogenin; unnamed protein product
Gene Aliases: bHLHc3; myf-4; MYF4; myo
UniProt ID: (Human) P15173, (Rat) P20428, (Mouse) P12979
Entrez Gene ID: (Human) 4656, (Rat) 29148, (Mouse) 17928
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
regulation of transcription, DNA-templated
muscle organ development
skeletal muscle tissue development
negative regulation of cell proliferation
tissue development
positive regulation of myotube differentiation
positive regulation of muscle atrophy
regulation of skeletal muscle satellite cell proliferation
response to muscle activity involved in regulation of muscle adaptation
response to electrical stimulus involved in regulation of muscle adaptation
striated muscle atrophy
response to denervation involved in regulation of muscle adaptation
skeletal muscle cell differentiation
muscle cell differentiation
muscle cell fate commitment
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
animal organ development
positive regulation of skeletal muscle fiber development
regulation of cell cycle
cellular response to estradiol stimulus
regulation of myoblast fusion
ossification
response to gravity
skeletal muscle atrophy
response to muscle activity
myotube differentiation
skeletal muscle tissue regeneration
myoblast differentiation
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
skeletal muscle fiber development
cellular response to magnetism
cellular response to lithium ion
cellular response to retinoic acid
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
positive regulation of oxidative phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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