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Invitrogen
NFAT5 Monoclonal Antibody (K49018_1C5)
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FIGURE: 1 / 1
NFAT5 Antibody (MA5-55769) in WB
Western blot analysis of NFAT5 in 15 µg of A549. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. NFAT5 band was visualized using ECL Substrate. Incubation with primary NFAT5 monoclonal antibody (Product # MA5-55769) at a dilution of 1 µg/mL was used.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-55769
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
K49018_1C5
Immunogen
Recombinant human NFAT5 (immunogen range is 271-540).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS with 50% glycerol, 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
Product Specific Information
Sequence of this protein is as follows: GQYPVKSEGK ELKIVVQPET QHRARYLTEG SRGSVKDRTQ QGFPTVKLEG HNEPVVLQVF VGNDSGRVKP HGFYQACRVT GRNTTPCKEV DIEGTTVIEV GLDPSNNMTL AVDCVGILKL RNADVEARIG IAGSKKKSTR ARLVFRVNIM RKDGSTLTLQ TPSSPILCTQ PAGVPEILKK SLHSCSVKGE EEVFLIGKNF LKGTKVIFQE NVSDENSWKS EAEIDMELFH QNHLIVKVPP YHDQHITLPV SVGIYVVTNA GRSHDVQPFT
Target Information
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutamine rich protein H65; NFAT; NFAT-like protein 1; NFAT5b; nuclear factor of activated T-cells 5, tonicity-responsive; osmotic response element-binding protein; T-cell transcription factor NFAT5; TonE-binding protein; TonEBP; transcription factor; tonicity-responsive enhancer-binding protein
Gene Aliases: NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
UniProt ID: (Human) O94916
Entrez Gene ID: (Human) 10725
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to stress
cellular response to DNA damage stimulus
signal transduction
calcineurin-NFAT signaling cascade
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
R-loop processing
positive regulation of mitochondrial translation
cellular response to cytokine stimulus
cellular hyperosmotic response
regulation of intracellular signal transduction
positive regulation of leukocyte adhesion to vascular endothelial cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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