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ATF6 Recombinant Mouse Monoclonal Antibody (8D3-R)
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FIGURE: 1 / 3
ATF6 Antibody (MA5-56440) in ICC/IF
Immunocytochemistry analysis of ATF6 with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with ATF6 Recombinant Monoclonal Antibody (Product # MA5-56440)at a dilution of 1:100 in 1% BSA in PBST overnight at 4 &(8)451;. Followed by Goat Anti-Mouse IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).
Product Details
MA5-56440
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8D3-R
Immunogen
Recombinant protein within Human ATF6 aa 1-670/670.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating transcription factor 6 alpha; Activating transcription factor 6 beta; ATF 6; ATF6 alpha; ATF6-alpha; ATF6-beta; cAMP response element binding protein-related protein; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; cAMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 beta; cAMP-responsive element-binding protein-like 1; CREB binding serum response factor; Creb-related protein; Creb-rp; Cyclic AMP-dependent transcription factor ATF-6 alpha; Cyclic AMP-dependent transcription factor ATF-6 beta; DKFZp686P2194; FLJ21663; Protein G13; unnamed protein product
Gene Aliases: 9130025P16Rik; 9630036G24; AA617266; AA789574; ACHM7; ATF6; ATF6A; Atf6alpha; ATF6B; ATF6beta; ATP6alpha; CREB-RP; CREBL1; ESTM49; G13
UniProt ID: (Human) P18850, (Human) Q99941, (Rat) G3V909, (Mouse) F6VAN0
Entrez Gene ID: (Human) 22926, (Human) 1388, (Rat) 304962, (Mouse) 226641, (Mouse) 12915, (Rat) 406169
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
enzyme binding
identical protein binding
sequence-specific DNA binding
protein heterodimerization activity
sequence-specific double-stranded DNA binding
cAMP response element binding
ubiquitin protein ligase binding
transcription regulatory region DNA binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to unfolded protein
signal transduction
endoplasmic reticulum unfolded protein response
response to endoplasmic reticulum stress
ATF6-mediated unfolded protein response
positive regulation of transcription from RNA polymerase II promoter
regulation of ATF6-mediated unfolded protein response
negative regulation of ATF6-mediated unfolded protein response
eye development
protein folding
visual perception
positive regulation of autophagy
ERAD pathway
positive regulation of apoptotic process
positive regulation of ATF6-mediated unfolded protein response
transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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