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Invitrogen
POLG Recombinant Rabbit Monoclonal Antibody (24GB1085)
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FIGURE: 1 / 4
POLG Antibody (MA5-57385) in ICC/IF
Immunocytochemical staining of POLG in HeLa cells using POLG Recombinant Monoclonal Antibody (Product # MA5-57385) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; POLG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium . Scale bar: 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57385
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB1085
Immunogen
A synthesized peptide derived from human DNA Polymerase gamma (aa 1190-1220)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exodeoxyribonuclease; 5'-deoxyribose-phosphate lyase; DNA polymerase gamma subunit 1 (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha); DNA-directed DNA polymerase gamma; FLJ27114; mitochondrial DNA polymerase catalytic subunit; mitochondrial DNA polymerase gamma; mitochondrial polymerase gamma; mitochondrial polymerase gamma catalytic subunit; Pol gamma; polG-alpha; polymerase (DNA directed), gamma; polymerase (DNA) gamma, catalytic subunit; polymerase, gamma; unnamed protein product
Gene Aliases: MIRAS; MTDPS4A; MTDPS4B; PEO; PolG-alpha; POLG1; POLGA; SANDO; SCAE
UniProt ID: (Human) P54098, (Rat) Q9QYV8
Entrez Gene ID: (Human) 5428, (Mouse) 18975, (Rat) 85472
protease binding
DNA binding
chromatin binding
DNA-directed DNA polymerase activity
protein binding
single-stranded DNA 3'-5' exodeoxyribonuclease activity
3'-5' exonuclease activity
DNA polymerase activity
5'-deoxyribose-5-phosphate lyase activity
exonuclease activity
catalytic activity, acting on a nucleic acid
DNA metabolic process
DNA replication
DNA-dependent DNA replication
mitochondrial DNA replication
base-excision repair
determination of adult lifespan
DNA replication proofreading
DNA biosynthetic process
base-excision repair, gap-filling
response to light stimulus
response to gamma radiation
response to hyperoxia
cellular response to glucose stimulus
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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