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Invitrogen

SMAD4 Recombinant Rabbit Monoclonal Antibody (22H12)

View all (73) SMAD4 antibodies

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Product Details

MA5-60677

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

22H12

Immunogen

A synthesized peptide derived from human SMAD4.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Amount

43 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3701300

Target Information

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; hSMAD4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD; SMAD, mothers against DPP homolog 4; unnamed protein product

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Gene Aliases: DPC4; JIP; MADH4; MYHRS

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UniProt ID: (Human) Q13485

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Entrez Gene ID: (Human) 4089

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding collagen binding filamin binding identical protein binding protein homodimerization activity sulfate binding sequence-specific DNA binding macromolecular complex binding SMAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding I-SMAD binding R-SMAD binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovarian follicle development osteoblast differentiation response to hypoxia epithelial to mesenchymal transition outflow tract septum morphogenesis cardiac conduction system development atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation left ventricular cardiac muscle tissue morphogenesis cardiac septum development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cellular iron ion homeostasis transforming growth factor beta receptor signaling pathway positive regulation of cell proliferation anatomical structure morphogenesis regulation of gene expression negative regulation of cardiac muscle hypertrophy positive regulation of gene expression epithelial cell migration positive regulation of cardiac muscle cell apoptotic process positive regulation of epithelial to mesenchymal transition cardiac muscle hypertrophy in response to stress cell differentiation negative regulation of cell growth adrenal gland development BMP signaling pathway positive regulation of transforming growth factor beta receptor signaling pathway regulation of transforming growth factor beta2 production activin receptor signaling pathway intracellular signal transduction atrioventricular canal development endothelial cell activation negative regulation of protein catabolic process embryonic digit morphogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter neuron fate specification positive regulation of SMAD protein import into nucleus SMAD protein signal transduction ventricular septum morphogenesis endocardial cell differentiation secondary palate development interleukin-6-mediated signaling pathway ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade cellular response to glucose stimulus cellular response to growth factor stimulus response to transforming growth factor beta cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus metanephric mesenchyme morphogenesis nephrogenic mesenchyme morphogenesis negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway transforming growth factor beta receptor superfamily signaling pathway positive regulation of extracellular matrix assembly positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac myofibril assembly positive regulation of cell proliferation involved in heart morphogenesis
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