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Invitrogen
Furin Recombinant Rabbit Monoclonal Antibody (25GB2160)
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FIGURE: 1 / 4
Furin Antibody (MA570976) in ICC/IF
Immunocytochemistry analysis of Furin in HeLa cells. Sample was incubated with Furin monoclonal antibody (Product # MA570975) at a dilution of 1:1,000. Nuclei were stained blue with DAPl and Alexa Fluor 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA570976
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
25GB2160
Immunogen
A synthesized peptide derived from human (210-350) Furin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.2 mg/mL
Amount
24 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
Target Information
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dibasic processing enzyme; dibasic-processing enzyme; FES upstream region; furin, membrane associated receptor protein; Paired basic amino acid cleaving enzyme (furin); paired basic amino acid residue cleaving enzyme; paired basic amino acid residue-cleaving enzyme; prohormone convertase 3; proprotein convertase subtilisin/kexin 3; proprotein convertase subtilisin/kexin type 3; Proprotein convertase subtilisin/kexin type 3 (paired basic amino acid cleaving enzyme, furin, membrane associated receptor protein); proprotein convertase subtilisin/kexin type3; subtilisin pro-protein processing enzyme; subtilisin-like proprotein convertase 1; unnamed protein product
Gene Aliases: 9130404I01Rik; FUR; PACE; PCSK3; SPC1
UniProt ID: (Human) P09958, (Rat) P23377, (Mouse) P23188
Entrez Gene ID: (Human) 5045, (Rat) 54281, (Mouse) 18550
protease binding
endopeptidase activity
serine-type endopeptidase activity
endopeptidase inhibitor activity
serine-type endopeptidase inhibitor activity
protein binding
heparin binding
peptidase activity
serine-type peptidase activity
obsolete signal peptidase activity
peptide binding
nerve growth factor binding
endopeptidase activator activity
heparan sulfate binding
blastocyst formation
proteolysis
regulation of signal transduction
protein processing
peptide hormone processing
viral life cycle
positive regulation of cell migration
positive regulation of transforming growth factor beta receptor signaling pathway
zymogen activation
regulation of cholesterol transport
negative regulation of low-density lipoprotein particle receptor catabolic process
nerve growth factor production
negative regulation of nerve growth factor production
negative regulation of transforming growth factor beta1 production
secretion by cell
regulation of protein catabolic process
cholesterol homeostasis
peptide biosynthetic process
protein maturation
dibasic protein processing
zymogen inhibition
cytokine precursor processing
negative regulation of inflammatory response to antigenic stimulus
transforming growth factor beta receptor signaling pathway
viral protein processing
extracellular matrix disassembly
extracellular matrix organization
collagen catabolic process
plasma lipoprotein particle remodeling
symbiosis, encompassing mutualism through parasitism
positive regulation of viral entry into host cell
positive regulation of membrane protein ectodomain proteolysis
amyloid fibril formation
viral entry into host cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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