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ApoA1 Monoclonal Antibody (513)
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FIGURE: 1 / 15
ApoA1 Antibody (MIA1404) in ICC/IF
Immunofluorescence analysis of Apolipoprotein A1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein A1 (513) Mouse Monoclonal Antibody (Product # MIA1404) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 c... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MIA1404
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
513
Immunogen
Purified human plasma Apo A-1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
5 mg/mL
Amount
1 mg
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
By sandwich ELISA, MIA1404 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MIA1404 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1404, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MIA1404 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1404 is also recommended for detecting endogenous Apo A-1 by Western blot.
MIA1404 was formerly sold as a Seradyn product.
Target Information
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apo a; apo A-1; APO A1; APO I; apo-AI; ApoAI; Apolipoprotein; apolipoprotein A1; apolipoprotein A1 homolog; Apolipoprotein AI; epididymis secretory sperm binding protein; MGC102525; MGC117399; MGC124929; proapo-A-I protein; proapolipoprotein; Udpgt; Ugt1; Ugt1a7; unnamed protein product
Gene Aliases: Alp-1; AMYLD3; apo(a); apo-AI; Apoa-1; apoA-I; Brp-14; HPALP2; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2
UniProt ID: (Human) P02647, (Mouse) Q00623
Entrez Gene ID: (Human) 335, (Mouse) 11806
beta-amyloid binding
receptor binding
lipid transporter activity
protein binding
phospholipid binding
high-density lipoprotein particle binding
lipid binding
cholesterol binding
enzyme binding
heat shock protein binding
apolipoprotein receptor binding
apolipoprotein A-I receptor binding
identical protein binding
protein homodimerization activity
chemorepellent activity
receptor agonist activity
phosphatidylcholine-sterol O-acyltransferase activator activity
high-density lipoprotein particle receptor binding
lipoprotein particle binding
intermembrane cholesterol transfer activity
lipase inhibitor activity
negative regulation of cytokine production involved in immune response
phosphatidylcholine biosynthetic process
lipid transport
transforming growth factor beta receptor signaling pathway
G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
cholesterol metabolic process
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of cholesterol efflux
negative regulation of very-low-density lipoprotein particle remodeling
protein oxidation
peptidyl-methionine modification
cholesterol transport
regulation of Cdc42 protein signal transduction
negative regulation of interleukin-1 beta production
cholesterol efflux
phospholipid efflux
negative regulation of heterotypic cell-cell adhesion
beta-amyloid formation
high-density lipoprotein particle remodeling
high-density lipoprotein particle assembly
high-density lipoprotein particle clearance
positive regulation of Rho protein signal transduction
lipoprotein metabolic process
cholesterol homeostasis
reverse cholesterol transport
negative regulation of inflammatory response
positive regulation of phagocytosis
protein stabilization
negative chemotaxis
vitamin transport
positive regulation of stress fiber assembly
acylglycerol homeostasis
phospholipid homeostasis
negative regulation of cell adhesion molecule production
negative regulation of response to cytokine stimulus
triglyceride homeostasis
ERK1 and ERK2 cascade
cholesterol import
cellular response to lipoprotein particle stimulus
positive regulation of cholesterol metabolic process
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of phospholipid efflux
regulation of protein phosphorylation
endothelial cell proliferation
phospholipid metabolic process
cholesterol biosynthetic process
glucocorticoid metabolic process
phospholipid transport
lipid storage
regulation of intestinal cholesterol absorption
adrenal gland development
organ regeneration
lipoprotein biosynthetic process
blood vessel endothelial cell migration
negative regulation of hydrolase activity
negative regulation of lipase activity
cellular response to beta-amyloid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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