Neon™ Transfection System - FAQs

We have tested four primary cell lines: HuVec, NHFF (Normal human foreskin fibroblast), MJ90 and NDHF. We have observed transfection efficiency in the range of 5-25%. We suggest optimizing transfection efficiency for your primary cell line by varying DNA and lipid ratio. If you are using any other primary cell line, please follow guidelines suggested in the product insert. Alternatively, Neon electroporation system and viral delivery methods are recommended.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

We have tested transfection of suspension cells such as Jurkat and K562. We achieved transfection efficiency of less than 5% for each of them. We do not notice toxicity with Lipofectamine LTX reagent when used with these suspension cells. For other cell lines please follow the guidelines in the product insert. However, if you experience low transfection efficiency, then we recommend Neon electroporation system or Viral delivery.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.