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Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer I

Catalog number:  N8080185

Related applications:

PCR

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Description

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:
  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it reliable for PCR applications
  • It is QC-tested to guarantee reproducible results

Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity: 5' - 3'
Hot Start: No
Product Line: AmpliTaq™
Quantity: 25,000 units
Fidelity (vs. Taq): 1 X
Overhang: 3'-A
Polymerase: AmpliTaq® DNA Polymerase
Reaction Format: Separate Components
Reaction Speed: Standard
Size (Final Product): 5 kb or less

Contents & storage

Contents:
  • 25 x 200 µL of AmpliTaq® DNA Polymerase (5 U/µL)
  • 25 x 1.5 mL of GeneAmp® 10X PCR Buffer I (100 mM Tris-HCl pH 8.3, 500 mM KCl, 15 mM MgCl2, 0.01% (w/v) gelatin)
Store at -20°C.