This product is designed for use with the new Mini Gel Tank ( A25977) or by retrofitting the Bolt Mini Gel Tank ( B4477599) with the Cassette Clamp Cam Handles Set ( A26732).

Bolt™ 10% Bis-Tris Plus Gels, 12-well

Catalog number: NW00102BOX

Invitrogen™  Related applications: Protein Gel Electrophoresis

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Description

Bolt® Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE® Bis-Tris gels, Bolt® Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt® gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt® Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt® Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank is required for use of all Bolt® gels.

Features include:
Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
Long shelf life—store gels for at least 16 months at 4–25°C
Better protein integrity—neutral pH sample preparation process preserves your proteins
Wedge-shaped wells—for easier sample loading and up to two times higher sample volume
Multi-channel pipettor compatible—achieve high throughput sample preparation and loading using the 17-well format

Choose the right Bolt® Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt® Bis-Tris Plus gels come in 4 polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt® MES or MOPS SDS running buffer to obtain different separation ranges. Bolt® Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt® Bis-Tris Plus gels also come in 4 different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt® Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt® MES running buffer. Bolt® Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than tris-glycine–based gels. In addition, Bolt® Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt® Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

New electrophoresis tank design
Bolt® Bis-Tris Plus gels are designed for use with the Mini Gel Tank. In addition, we recommend the use of Bolt® MES or MOPS SDS running buffers with these gels.

Note: Bolt® Bis-Tris Plus gels, once removed from the cassette, are compatible with the Mini Blot Module and iBlot® 2 Gel Transfer Device for efficient and reliable western blot transfer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Percentage: 10 %
Wells: 12 wells
Gel Type: Bis-Tris Plus Gel
Separation Range: 3.5 kDa to 160 kDa (MES Buffer), 15 kDa to 260 kDa (MOPS Buffer)
Gel Size: Mini (8 cm x 8 cm)
Well Format: 1D Well (Wedge-Shaped)
Thickness: 1.0 mm
Adapters: No Adapters
Capacity: Up to 45 µL per well
Quantity: 10 gels
Product Size: 10 gels (1 box)
Mode of Separation: Molecular Weight
Sample Loading Volume: 30 µL (recommended)
For Use With (Equipment): Mini Gel Tank
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

One box contains 10 gels. Store at 4–25°C. Shelf life is 16 months.

Documents

Manuals & protocols

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