El ácido carboxílico Oregon Green™ 488, éster de succinimidilo aminorreactivo puede usarse para crear bioconjugados de fluorescencia verde con excitación/emisiónMás información
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Número de catálogo
Cantidad
O6149
5 mg
Número de catálogo O6149
Precio (MXN)
-
Cantidad:
5 mg
El ácido carboxílico Oregon Green™ 488, éster de succinimidilo aminorreactivo puede usarse para crear bioconjugados de fluorescencia verde con excitación/emisión máxima de ∼496/524 nm. Este análogo fluorado de la fluoresceína supera algunas de las limitaciones clave de la fluoresceína, incluidas una mayor fotoestabilidad y una pKa más baja (pKa ∼4,7 frente a 6,4 de la fluoresceína), lo que hace que su fluorescencia sea esencialmente insensible al pH en el intervalo de pH fisiológico.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Emisión524
Excitación496
Etiqueta o tinteOregon Green™ 488
Tipo de productoÁcido carboxílico Éster de succinimidilo
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (49)
Citations & References
Abstract
The dystroglycan complex is necessary for stabilization of acetylcholine receptor clusters at neuromuscular junctions and formation of the synaptic basement membrane.
Authors:Jacobson C, Côté PD, Rossi SG, Rotundo RL, Carbonetto S
Journal:J Cell Biol
PubMed ID:11157973
'The dystrophin-associated protein (DAP) complex spans the sarcolemmal membrane linking the cytoskeleton to the basement membrane surrounding each myofiber. Defects in the DAP complex have been linked previously to a variety of muscular dystrophies. Other evidence points to a role for the DAP complex in formation of nerve-muscle synapses. We ... More
Larval Schistosoma mansoni excretory-secretory glycoproteins (ESPs) bind to hemocytes of Biomphalaria glabrata (Gastropoda) via surface carbohydrate binding receptors.
Authors:Johnston LA, Yoshino TP
Journal:J Parasitol
PubMed ID:11534642
'Flow cytometric analysis of circulating blood cells (hemocytes) of Biomphalaria glabrata, molluscan intermediate host of Schistosoma mansoni, revealed the presence of 2 overlapping hemocyte subpopulations, designated R1 and R2. R1 hemocytes are characterized by their smaller size, reduced granularity, and the presence of the BGH1 surface epitope, whereas R2 cells ... More
Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages.
Authors:Porte F, Liautard JP, Köhler S
Journal:Infect Immun
PubMed ID:10417172
'Brucella suis is a facultative intracellular pathogen of mammals, residing in macrophage vacuoles. In this work, we studied the phagosomal environment of these bacteria in order to better understand the mechanisms allowing survival and multiplication of B. suis. Intraphagosomal pH in murine J774 cells was determined by measuring the fluorescence ... More
Acidification of the phagosome in Crassostrea virginica hemocytes following engulfment of zymosan.
Authors:Beaven AE, Paynter KT
Journal:Biol Bull
PubMed ID:10065529
'Phagocytic hemocytes are responsible for engulfing and internally degrading foreign organisms within the hemolymph and tissue of the eastern oyster, Crassostrea virginica. Since rapid acidification of the phagosome lumen is typically essential for activation of hydrolytic and reactive oxygen intermediate (ROI) producing enzymes in vertebrate cells, we measured phagosomal pH ... More
A comparison of the emission efficiency of four common green fluorescence dyes after internalization into cancer cells.
Authors:Hama Y, Urano Y, Koyama Y, Bernardo M, Choyke PL, Kobayashi H
Journal:Bioconjug Chem
PubMed ID:17105220
'In vivo optical imaging to enhance the detection of cancer during endoscopy or surgery requires a targeted fluorescent probe with high emission efficiency and high signal-to-background ratio. One strategy to accurately detect cancers is to have the fluorophore internalize within the cancer cells permitting nonbound fluorophores to be washed away ... More