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Pacific Blue™ Antibody Labeling Kit, 5 Reactions kit - FAQs

Pacific Blue™ Antibody Labeling Kit, 5 Reactions kit - FAQs

View additional product information for Pacific Orange™ and Pacific Blue™ Antibody Labeling Kits - FAQs (P30013, P30014)

2 product FAQs found

What formulation of antibody should I use for conjugation for small animal in vivo imaging?

To allow for good reaction kinetics, antibodies should be in PBS buffer at a concentration of 0.5-3.0 mg/ml. The antibody must be free of preservatives (azide etc.), amine containing buffers and carrier proteins such as BSA.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is degree of labeling (DOL)?

Degree of labeling (DOL) describes the number of fluorophores per antibody. For in vivo labeling experiments, the DOL is restricted to a narrow range because it has significant consequences for the biodistribution and clearance of the probe. For example, for in vivo imaging, we have determined that the DOL range for the far-red Alexa Fluor dyes is 1.5 to 3 molecules per antibody for optimal optical in vivo imaging.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.