pHrodo™ Deep Red Antibody Labeling Kit, 3 x 100 μg Reactions

pHrodo™ Deep Red Antibody Labeling Kit, 3 x 100 μg Reactions - FAQs

View additional product information for pHrodo™ Antibody Labeling Kits - FAQs (P36020, P36023, P36022, P36021, P35356, P35355, P36015, P36014)

3 product FAQs found

May I store antibodies or other proteins labeled with the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) in solutions containing sodium azide?

We do not recommend storing antibodies or other proteins labeled with the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) in solutions containing sodium azide, as pHrodo Deep Red is not stable in the presence of sodium azide. Consider using an alternative, non-azide-based anti-microbial.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do I really need to do a DOL determination when using pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356)?

A DOL determination is required to determine if a suitable amount of dye is attached to the protein. Without a DOL determination, you would not be able to determine if the protein was adequately labeled. A low fluorescent signal may be the same for a protein that is under-labeled or over-labeled, as over-labeling may cause some level of dye-dye quenching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May I use the pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) to label other proteins that are not antibodies?

Yes. The pHrodo Deep Red Antibody Labeling Kit (Cat. Nos. P35355, P35356) was optimized to label either 100 µg or 1 mg of a ~150 kDa protein (molecular weight of a whole IgG) but can be used to label other proteins. Optimize the mass of protein to label based on the molecular weight of the protein relative to the molecular weight of a whole IgG (~150 kDa).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.